Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

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7.8K Views

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08:56 min

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June 27th, 2017

DOI :

10.3791/55505-v

June 27th, 2017

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Keiji Hirai¹, Hiromichi Yoshizawa², Toshimi Imai², Yusuke Igarashi², Ichiro Hirahara², Susumu Ookawara², Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ²Division of Nephrology, Department of Internal Medicine, Jichi Medical University, ³Department of Medical Physiology, Meiji Pharmaceutical University

Podsumowanie

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Keyword Extraction MiRNA

Peritoneal Membrane

Quantitative Real time PCR

Rozdziały w tym wideo

0:05

Title

0:56

Peritoneum Sample Collection

8:29

Conclusion

8:06

Results: Upregulated miRNAs in the Peritoneal Membrane of Peritoneal Fibrosis Rats

1:54

Purifying Total RNAs from Peritoneal Membrane Samples

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Reverse Transcription of Total RNA

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qRT-PCR of miRNA

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