JoVE Journal

Genetics

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Nanyang Technological University

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

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8.1K Views

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08:22 min

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September 15th, 2018

DOI :

10.3791/57920-v

September 15th, 2018

•

Fani Memi¹, Daniela Tirziu², Irinna Papangeli³

¹Department of Cell and Developmental Biology, University College London, ²Yale Cardiovascular Research Group, Section of Cardiovascular Medicine, Yale School of Medicine, ³Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Yale School of Medicine

Podsumowanie

micro-RNAs (miRNAs) are short and highly homologous RNA sequences, serving as post-transcriptional regulators of messenger RNAs (mRNAs). Current miRNA detection methods vary in sensitivity and specificity. We describe a protocol that combines in situ hybridization and immunostaining for concurrent detection of miRNA and protein molecules on mouse heart tissue sections.

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Tissue specific

In Situ Hybridization

Protein Detection

Rozdziały w tym wideo

0:04

Title

0:24

Tissue Preparation

2:07

Probe Hybridization

3:55

DIG Antibody Detection

5:20

cTNT Antibody Detection and DAPI Staining

6:25

Results: PIGF-overexpressing Heart Tissue and miRNA-182 Expression

7:33

Conclusion

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