Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

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29.1K Views

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08:37 min

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August 27th, 2016

DOI :

10.3791/54358-v

August 27th, 2016

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F. Javier Rubio*¹, Xuan Li*¹, Qing-Rong Liu¹, Raffaello Cimbro², Bruce T. Hope¹

¹Behavioral Neuroscience Research Branch, Intramural Research Program, NIDA, NIH, DHHS, ²Division of Rheumatology, School of Medicine, Johns Hopkins University

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Rozdziały w tym wideo

0:05

Title

0:43

Tissue Collection and Dissection

2:17

Cell Dissociation, Fixation and Permeabilization

5:52

Results: Representative Flow Cytometric and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Brain Tissue

7:16

Conclusion

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