Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

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8.2K Views

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15:29 min

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August 5th, 2016

DOI :

10.3791/54263-v

August 5th, 2016

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Alain Casanova*¹, Shyan H. Low*¹, Mario Emmenlauer*¹^,⁴, Raquel Conde-Alvarez*¹^,³, Suzana P. Salcedo², Jean-Pierre Gorvel², Christoph Dehio¹

¹Focal Area Infection Biology, Biozentrum, University of Basel, ²Centre d’Immunologie de Marseille-Luminy, Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, ³Departmento de Microbiologìa and Instituto de Salud Tropical, Universidad de Navarra, ⁴BioDataAnalysis GmbH

Podsumowanie

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Keywords Microscopy

High throughput Screening

Rozdziały w tym wideo

0:05

Title

0:54

Reverse siRNA Transfection

3:19

Infection and Fixation

5:55

Staining and Imaging

8:14

Automated Image Analysis

12:21

Results: Automated High-throughput Quantification of Brucella Infection in HeLa Cells

14:06

Conclusion

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