JoVE Journal

Neuroscience

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Nanyang Technological University

Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis

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26.5K Views

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09:24 min

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April 22nd, 2015

DOI :

10.3791/52551-v

April 22nd, 2015

•

Anne Ansorg*¹, Katja Bornkessel*¹, Otto W. Witte¹, Anja Urbach¹

¹Hans Berger Department of Neurology, Jena University Hospital

Podsumowanie

This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

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Keywords Adult Neurogenesis

Immunohistochemistry

Multiple Labeling

Neural Stem Cells

Progenitor Subtypes

Proliferation Markers

Immunofluorescence

Confocal Microscopy

Cell Quantification

Sequential Immunofluorescence

Rozdziały w tym wideo

0:05

Title

2:17

Injection of Thymidine Analogs and Tissue Preparation

3:38

Sequential Multiple-Immunofluorescence with Primary Antibodies from the Same Host Species

6:27

Imaging and Data Analysis

7:05

Newborn Hippocampal Cells in Cyclin D2 Deficient Mice

8:42

Conclusion

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