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Neuroscience

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Nanyang Technological University

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders

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11.2K Views

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07:43 min

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May 12th, 2015

DOI :

10.3791/52919-v

May 12th, 2015

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Giovanni Provenzano¹, Luca Pangrazzi¹, Andrea Poli², Nicoletta Berardi², Yuri Bozzi¹^,²

¹Centre for Integrative Biology (CIBIO), University of Trento, ²CNR Neuroscience Institute, Pisa, Italy

Podsumowanie

We describe an immunohistochemistry protocol to study the profile of hippocampal neuron activation following exposure to a spatial learning task in a mouse model characterized by cognitive deficits of neurodevelopmental origin. This protocol can be applied to both genetic or pharmacological mouse models characterized by cognitive deficits.

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Immunohistochemistry

Spatial Learning

Morris Water Maze

Engrailed 2 Knockout

Autism Spectrum Disorders

Neurodevelopmental Disorders

Neuronal Activation

Rozdziały w tym wideo

0:05

Title

1:30

Preparation of Brain Sections

2:16

pERK Immunohistochemistry

5:22

Bright-field Imaging of Neuron Activation

5:43

Results: pERK is a Reliable Molecular Readout of Learning Dependent Neuronal Activation

6:45

Conclusion

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