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Neuroscience

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Nanyang Technological University

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

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16.8K Views

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08:37 min

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May 28th, 2015

DOI :

10.3791/52537-v

May 28th, 2015

•

Jaclyn M. Schwarz¹

¹Department of Psychological and Brain Sciences, University of Delaware

Podsumowanie

The goal of this protocol is to use the fluorescence activated cell sorting (FACS) technique to sort specific types of neural cells for subsequent analysis of cell-type-specific gene expression, epigenetic markers, and or protein expression.

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Keywords Fluorescence Activated Cell Sorting

Cell type specific Gene Expression

Rat Brain Tissue

Oligodendrocytes

Cell type specific Analysis

Neural Function

Behavioral Epigenetics

Gene Expression

Protein Expression

Cell to cell Communication

Rozdziały w tym wideo

0:05

Title

1:09

Neural Dissociation

3:14

Myelin Depletion

4:50

FACS Staining

6:27

Results: Sorting Neurons, Astrocytes, and Microglia from Individual Samples

8:11

Conclusion

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