JoVE Journal

Neuroscience

This content is Open Access.

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Please note that all translations are automatically generated. Click here for the English version.

14.1K Views

•

09:27 min

•

September 14th, 2015

DOI :

10.3791/53247-v

September 14th, 2015

•

Mathieu Daynac*¹^,²^,³^,⁴^,⁵, Lise Morizur*¹^,²^,³^,⁴, Thierry Kortulewski¹^,²^,³^,⁴, Laurent R. Gauthier¹^,²^,³^,⁴, Martial Ruat⁵, Marc-André Mouthon¹^,²^,³^,⁴, François D. Boussin¹^,²^,³^,⁴

¹CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, ²INSERM, UMR 967, ³Université Paris Diderot, Sorbonne Paris Cité, UMR 967, ⁴Université Paris Sud, UMR 967, ⁵CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department

Podsumowanie

We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging.

Przeglądaj więcej filmów

Neural Stem Cells

Subventricular Zone

Fluorescence activated Cell Sorting FACS

Cell Cycle Dynamics

Transit Amplifying Cells

Rozdziały w tym wideo

0:05

Title

1:38

Basic Setup

2:50

SVZ Tissue Dissociation

4:19

Immunofluorescent Staining and Cell Sorting

6:21

Microscope Setup and Image Acquisition

7:57

Results: Live Imaging of the Cell Cycle Phases at the Level of a Single Cell

8:53

Conclusion

Powiązane Filmy

article

The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

41.8K Views

article

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

22.0K Views

article

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

12.0K Views

article

The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

15.4K Views

article

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

13.3K Views

article

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

17.1K Views

article

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

15.2K Views

article

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

14.1K Views

article

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

15.4K Views

article

Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone

15.1K Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej