Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

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16.6K Views

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09:49 min

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October 8th, 2013

DOI :

10.3791/50696-v

October 8th, 2013

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Ludovico Silvestri¹, Alessandro Bria²^,³, Irene Costantini¹, Leonardo Sacconi¹^,⁴, Hanchuan Peng⁵, Giulio Iannello², Francesco Saverio Pavone¹^,⁴^,⁶^,⁷

¹European Laboratory for Non-linear Spectroscopy (LENS), ²Integrated Research Centre, University Campus Bio-medico of Rome, ³DAEMI, University of Cassino, ⁴National Institute of Optics (CNR-INO), ⁵Allen Institute for Brain Science, ⁶Department of Physics, University of Florence, ⁷ICON Foundation, Sesto Fiorentino, Italy

Podsumowanie

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Keywords Optical Tomography

Confocal Light Sheet Microscopy

CLSM

Entire Mouse Brain

Micron scale Resolution

Multi resolution Navigation

Rozdziały w tym wideo

0:05

Title

1:22

Dehydration and Clearing

2:37

Specimen Mounting

3:33

Tomography Preparation

4:26

Tomography Acquisition

5:37

Stitching and Visualization

7:35

Results: Micron-scale Resolution Optical Tomography

8:54

Conclusion

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