Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

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1.9K Views

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11:12 min

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December 1st, 2021

DOI :

10.3791/63308-v

December 1st, 2021

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Nikita V. Zhilyakov¹, Arsenii Y. Arkhipov¹, Eduard F. Khaziev¹^,², Marat A. Mukhamedyarov³, Dmitry V. Samigullin¹^,²

¹Laboratory of Biophysics of Synaptic Processes, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, ²Department of Radiophotonics and Microwave Technologies, Kazan National Research Technical University named after A.N. Tupolev, ³Department of Normal Physiology, Kazan State Medical University

Podsumowanie

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Calcium Transients

Neuromuscular Junction

High Temporal Resolution

Confocal Microscopy

Synaptic Transmission

Neurodegenerative Diseases

Calcium Signaling

Loading Technique

Neurophysiology

Muscle Tissue Fixation

Micropipette Preparation

Intracellular Recordings

Nerve Branches

Dye Loading Solution

Ringer s Solution

Rozdziały w tym wideo

0:05

Introduction

1:12

Dye Loading Procedure

3:28

Video Capture with Confocal Microscopy

6:14

Video Processing

7:56

Video Analysis

9:55

Results: Mouse Nerve Terminals Loaded with the Ca²⁺ Indicator and Representative Trace of Calcium Signal

10:18

Conclusion

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