Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
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14:58 min
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November 12th, 2012
DOI :
November 12th, 2012
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Rozdziały w tym wideo
0:05
Title
1:47
Construction of Gene-specific SPA-tagging in E. Coli DY330 Strain
3:54
Culturing and Sonication
5:26
Affinity Purification
7:53
Proteolysis and Sample Preparation for Mass Spectrometry
9:38
Protein Identification by LTQ Orbitap Velos Mass Spectrometer
11:02
Results
14:27
Conclusion
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