The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

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41.7K Views

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14:33 min

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May 6th, 2010

DOI :

May 6th, 2010

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Zaman Mirzadeh¹, Fiona Doetsch²^,³, Kazunobu Sawamoto⁴, Hynek Wichterle²^,⁵, Arturo Alvarez-Buylla¹

¹Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco - UCSF, ²Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, ³Department of Neuroscience and Neurology, College of Physicians and Surgeons, Columbia University, ⁴Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, ⁵Center for Motor Neuron Biology and Disease, College of Physicians and Surgeons, Columbia University

Podsumowanie

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Subventricular Zone

Wholemount Staining

Lateral Ventricles

Germinal Region

Neural Stem Cells

Adult Neurogenesis

Wholemount Technique

Histological Analysis

Cytoarchitecture

Cellular Relationships

Neuroepithelium

Planar Polarization

Cerebrospinal Fluid Flow

Stem Cell Niche

Rozdziały w tym wideo

0:00

Title

0:07

Introduction

1:02

Wholemount Dissection

4:14

Ependymal Flow Analysis Using Fluorescent Microbeads

6:41

Fixing and Immunostaining Wholemounts

10:58

Representative Images and Ependymal Flow Data

12:20

Conclusion

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