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Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors

1.9K Views

08:16 min

July 27th, 2022

DOI :

10.3791/63837-v

July 27th, 2022


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Single Molecule Analysis

Hoofdstukken in deze video

0:05

Introduction

1:22

P0 Virus Stock Generation

2:38

P1 Virus Stock Generation and Large-Scale Infection

3:28

Sf9 Purification of Kinesin-3 Motors

4:25

Microtubule Polymerization and In Vitro Single-Molecule Motility Assay

5:22

In Vitro Microtubule Gliding Assay

6:23

Results: Analysis of Sf9-Purified Kinesin-3 Motor

7:24

Conclusion

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