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Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

15.7K Views

12:47 min

August 29th, 2017

DOI :

10.3791/56186-v

August 29th, 2017


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Chromatin Immunoprecipitation ChIP

Hoofdstukken in deze video

0:05

Title

0:57

Preparation of Eggs and Microdissection of Chicken Spinal Neural Tube (SNT)

4:12

Crosslinking Proteins to DNA

5:01

Lysis, Sonication, and Immunoprecipitation

6:33

Washing, Elution, and Reversal of Crosslinking

8:57

Cellular Protein and RNA Digestion

10:06

Results: Evaluation of the ChIP Protocol by ChIP-seq Experiments

11:48

Conclusion

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