데이터의 수집과 관련하여 도움이 박사 란 진과 쉐인 Stanek에 저자 감사합니다.

<ol>
	<li>Low, P. A., Denq, J. C., Opfer-Gehrking, T. L., Dyck, P. J., O'Brien, P. C., Slezak, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+age+and+gender+on+sudomotor+and+cardiovagal+function+and+blood+pressure+response+to+tilt+in+normal+subjects.">Effect of age and gender on sudomotor and cardiovagal function and blood pressure response to tilt in normal subjects.</a> Muscle Nerve. 20, 1561-1568 (1997).</li><li>Low, P. A., Denq, J. C., Opfer-Gehrking, T. L., Dyck, P. J., O'Brien, P. C., Slezak, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+age+and+gender+on+sudomotor+and+cardiovagal+function+and+blood+pressure+response+to+tilt+in+normal+subjects.">Effect of age and gender on sudomotor and cardiovagal function and blood pressure response to tilt in normal subjects.</a> Muscle Nerve. 20, 1561-1568 (1997).</li><li>Low, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Composite+autonomic+scoring+scale+for+laboratory+quantification+of+generalized+autonomic+failure.">Composite autonomic scoring scale for laboratory quantification of generalized autonomic failure.</a> Mayo Clin Proc. 68, 748-752 (1993).</li><li>Low, P. A., Low, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laboratory+evaluation+of+autonomic+function.">Laboratory evaluation of autonomic function.</a> Clinical Autonomic disorders. , 179-209 (1997).</li><li>Low, P. A., Low, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laboratory+evaluation+of+autonomic+failure.">Laboratory evaluation of autonomic failure.</a> Clinical Autonomic disorders. , 179-209 (2008).</li><li>Novak, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+sympathetic+index+from+Valsalva+maneuver.">Assessment of sympathetic index from Valsalva maneuver.</a> Neurology. , (2011).</li></ol>

관심 없음 충돌 선언하지 않습니다.

주의할 점 자율 테스트를 완료하는 것은 간단 환자의 대부분에 1 시간 할 수 있습니다. 테스트의 각 단계의 특정 함정은 아래 설명되어 있습니다. 장비, 자율 신호의 데이터 수집 및 가공. 주로 데이터 품질에 관한 함정의 숫자가 있습니다. 피부의 불충 분한 청소 R 파의 검출이 어려운 렌더링할 수 ECG 신호의 과도한 소음으로 이어질 수 있습니다. 혈압 센서 내의 부적 절한 배치 허위 높거나 낮은 값을 제공합니다. 양질의 Transcranial 도플러 신호는 작은 뼈 창 인해 노인 환자에 들어갈 어려울 수 있습니다. 데이터 처리를위한 소프트웨어는 진동과 파킨슨 환자의 예를 들어, 혈압에서 유물을 구별할 수있을만큼 강력한하지 않을 수 있습니다. 심호흡 <ol><li> 불안과 비정상적인 호흡 패턴은 깊은 호흡의 결과에 영향을 줄 수 있습니다. 제목은 테스트 기간 동안 잘 편안한해야합니다. 불안의 좋은 마커는 심장 박동 프로필입니다. 피사체가 불안 경우, 심장 박동은 고가 수 있습니다. 심호흡 테스트를 시작하기 전에, 의미 심장 박동이 평평하게 유지 때까지 기다 된 경우 심장 박동이 위 또는 아래로 동향되지 않았는지 확인합니다. 쉬고 호흡 패턴이 정상되어야합니다, 예를 들어 더 hyperventilation합니다 (끝 갯벌 CO 2에 의해 측정) 또는 tachypnea (호흡기 속도가 분당 11 위에있다)가 없어야합니다. </li><li> 심장의 vagal 신경 변조 마음 생성한 리듬에 의해 또는 맥박 조정기에 의해 무시되기 때문에 깊은 호흡 시험은 심방 세동 또는 때 심장 박동은 맥박 조정기에 의해 제어됩니다 예를 특정 심장 dysrythmia 제한 유용있다. 심장 리듬의 이상의 기본 지식을 권장합니다. </li><li> R 파도로 인해 유물의 부적 절한 감지 분명 결과에 오류가 발생할 것입니다. 이것은 R의 파도가 안정적으로 감지 너무 작은 일이있다면, 또는 ECG 배경 예를 들면 시끄러운 경우로 인해 가난한 피부 문의할 수 있습니다. </li></ol> Valsalva 책략 Valsalva의 작전 대상에서 대부분의 노인이나 연약한 환자가 제대로 Valsalva의 책략을 수행할 수 없습니다 상당한 협력이 필요합니다. 가장 일반적인 이유는 입 근육의 약점이다 호흡기 부담 동안 공기 누출로 이어지는. 이 경우는 손가락 6 관 주위 인감 공기 누출을 부드럽게하는 효과가있다. 아드레날린의 기능 평가 Valsalva 책략 (그림 10)의 평방 파도 변형에 할 수 없습니다. 이 응답은 드물지만 건강한 과목 또는 울혈 심장 마비 환자에서 볼 수 있습니다. 틸트 테스트 약물 번호 어려운 시험의 기울기 테스트 렌더링 해석에 영향을 줄 수 있습니다. 그것은 테스트하기 전에 약물을 중단하는 것이 실용적되지 않습니다. 그것은 진동과 파킨슨 환자의 예를 들어, 운동 장애 환자에서 양질의 녹음을 얻기 어려울 수 있습니다. Sudomotor 테스트 (QSART) <ol><li> 기름 표면 또는 너무 많은 피부 자극의 불완전한 제거 땀을 출력에 영향을 줄 수 있습니다. </li><li> 누출 캡슐 인해 너무 꽉 캡슐 방수이나 통증이되지 않는 경우 </li><li> 일반적으로 사용되는 약물은 땀을 시험을 방해 anticholinergic 효과가 있습니다. 대부분의 경우에는 어려운 결과 이​​러한 약물 렌더링 해석을 중단할 허무 있습니다. </li></ol> 복합 자율 심각도 평가 점수 (카스)를 사용 자율 장애의 등급. 그것이 절대 및 퍼센트 값을 모두 여러 비교를 포함하기 때문에 카스 점수의 생성은 아주 복잡합니다. 불행히도, 자동으로 점수를 계산하기 위해 수도 상용 소프트웨어가 없습니다. 매사 추세츠에서, 우리는 자동으로 카스 점수를 생성 독점 소프트웨어를 사용합니다. 카스 점수의 예는 표 8에 있습니다.

표 9 추천 장비. <table border="1"><tbody><tr><td> 장비의 명칭 </td><td> 회사 </td><td> 댓글 </td></tr><tr><td> 전동 틸트 테이블 </td><td> WR 의료 전자 </td><td> 5-10초 이내에 70 정도에 도달 부드러운 움직임과 함께 모든 테이블에 </td></tr><tr><td> ECG 모니터 </td><td></td><td> 아날로그 출력을 가진 장치 </td></tr><tr><td> 비트 - 투 - 비트의 혈압 모니터 비침 투 </td><td> Finapres 의료 장비 </td><td> 몇 가지 모델을 사용할 수 있으며, 모두가 비슷합니다 </td></tr><tr><td> Q - 스웨트 </td><td> WR 의료 전자 </td><td></td></tr><tr><td> Transcranial 도플러 </td><td></td><td> 아날로그 출력을 가진 장치 </td></tr><tr><td> 레코딩 시스템 </td><td> LabChart, ADInstruments </td><td> 간단한 프로그래밍 </td></tr><tr><td> 레코딩 시스템 </td><td> Labview </td><td> 복잡한 프로그래밍 </td></tr><tr><td> 레코딩 시스템 </td><td> Matlab </td><td> 복잡한 프로그래밍 </td></tr><tr><td> 레코딩 시스템 </td><td> WR 의료 </td><td> 사용할 수 있지만, 제한된 유연성 </td></tr><tr><td> CO2 모니터 </td><td></td><td> 수면 연구에 사용되는 대부분의 모델 </td></tr><tr><td> 호흡기 모니터 </td><td></td><td> 간단한 서미 스터는 기록 시스템에 부착된 </td></tr></tbody></table>

quantitative autonomic testing

자율 신경계의 기능 장애와 관련된 장애는 매우 흔한 아직 자주 인식할 수 있습니다. 정량 자율 테스트는 임상 연구 모두에서 이러한 장애의 평가를위한 귀중한 도구가 될 수 있습니다. 자율 검사 숫자가있다, 그러나, 몇 가지는 임상적으로 확인 또는 정량 아르했다. 여기, 자율 기능의 테스트에 대해 전적으로 양적 및 임상 검증 프로토콜이 제공됩니다. 최소한으로 임상 자율 연구소는 틸트 테이블, ECG 모니터, 지속적인 비침 투 혈압 모니터, 호흡 모니터 및 sudomotor 도메인의 평가에 대해 의미가 있어야합니다. 자율 테스트 기록 및 평가를위한 소프트웨어는 데이터의 정확한 평가를 위해 중요합니다. cardiovagal, 아드레날린과 sudomotor : 제시 프로토콜은 3 대 자율 도메인을 평가합니다. 테스트는 심호흡, Valsalva의 기동, 헤드 업 틸트 및 양적 sudomotor의 축삭 테스트 (QSART)를 포함합니다. 심각하고 dysautonomia의 분포는 복합 자율 심각도 점수 (카스)를 사용하여 quantitated입니다. 상세 프로토콜 관련 매개 변수와 자율 신호의 불편 평가를 획득, 적절한 데이터 수집에 중점와 함께 테스트를 필수적인 측면을 강조 제공됩니다. 결과의 해석에 대한 규범적인 데이터와 카스 알고리즘은 물론 제공됩니다.

Watch this Scientific Journal Video about Quantitative Autonomic Testing at JoVE.com

Quantitative Autonomic Testing

자율 기능의 표준화, 종합적이고 완벽하게 정량 시험이 설명되어 있습니다. 자율 검사 cardiovagal, 아드레날린 및 sudomotor 포함한 모든 세 가지 주요 자율 도메인의 평가로 구성되어 있습니다. 심각하고 dysautonomia의 분포는 복합 자율 심각도 점수를 사용하여 quantitated 있습니다.

양적 자율 테스트

Disorders associated with dysfunction of autonomic nervous system are quite common yet frequently unrecognized. Quantitative autonomic testing can be ...

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56.4K 조회수.  University of Massachusetts Medical School. 자율 기능의 표준화, 종합적이고 완벽하게 정량 시험이 설명되어 있습니다. 자율 검사 cardiovagal, 아드레날린 및 sudomotor 포함한 모든 세 가지 주요 자율 도메인의 평가로 구성되어 있습니다. 심각하고 dysautonomia의 분포는 복합 자율 심각도 점수를 사용하여 quantitated 있습니다.

비디오: Quantitative Autonomic Testing

Quantitative Analysis of Chromatin Proteomes in Disease

In the nucleus reside the proteomes whose functions are most intimately linked with gene regulation. Adult mammalian cardiomyocyte nuclei are unique due to the high percentage of binucleated cells,1 the predominantly heterochromatic state of the DNA, and the non-dividing nature of the cardiomyocyte which renders adult nuclei in a permanent state of interphase.2 Transcriptional regulation during development and disease have been well studied in this organ,3-5 but what remains relatively unexplored is the role played by the nuclear proteins responsible for DNA packaging and expression, and how these proteins control changes in transcriptional programs that occur during disease.6 In the developed world, heart disease is the number one cause of mortality for both men and women.7 Insight on how nuclear proteins cooperate to regulate the progression of this disease is critical for advancing the current treatment options.
Mass spectrometry is the ideal tool for addressing these questions as it allows for an unbiased annotation of the nuclear proteome and relative quantification for how the abundance of these proteins changes with disease. While there have been several proteomic studies for mammalian nuclear protein complexes,8-13 until recently14 there has been only one study examining the cardiac nuclear proteome, and it considered the entire nucleus, rather than exploring the proteome at the level of nuclear sub compartments.15 In large part, this shortage of work is due to the difficulty of isolating cardiac nuclei. Cardiac nuclei occur within a rigid and dense actin-myosin apparatus to which they are connected via multiple extensions from the endoplasmic reticulum, to the extent that myocyte contraction alters their overall shape.16 Additionally, cardiomyocytes are 40% mitochondria by volume17 which necessitates enrichment of the nucleus apart from the other organelles. Here we describe a protocol for cardiac nuclear enrichment and further fractionation into biologically-relevant compartments. Furthermore, we detail methods for label-free quantitative mass spectrometric dissection of these fractions-techniques amenable to in vivo experimentation in various animal models and organ systems where metabolic labeling is not feasible.

Advances in mass spectrometry have allowed the high throughput analysis of protein expression and modification in a host of tissues. Combined with subcellular fractionation and disease models, quantitative mass spectrometry and bioinformatics can reveal new properties in biological systems. The method described herein analyzes chromatin-associated proteins in the setting of heart disease and is readily applicable to other in vivo models of human disease.

질병의 염색질 Proteomes의 정량 분석

In the nucleus reside the proteomes whose functions are most intimately linked with gene regulation. Adult mammalian cardiomyocyte nuclei are unique due ...

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Measuring Cardiac Autonomic Nervous System (ANS) Activity in Children

The autonomic nervous system (ANS) controls mainly automatic bodily functions that are engaged in homeostasis, like heart rate, digestion, respiratory rate, salivation, perspiration and renal function. The ANS has two main branches: the sympathetic nervous system, preparing the human body for action in times of danger and stress, and the parasympathetic nervous system, which regulates the resting state of the body.
ANS activity can be measured invasively, for instance by radiotracer techniques or microelectrode recording from superficial nerves, or it can be measured non-invasively by using changes in an organ's response as a proxy for changes in ANS activity, for instance of the sweat glands or the heart. Invasive measurements have the highest validity but are very poorly feasible in large scale samples where non-invasive measures are the preferred approach. Autonomic effects on the heart can be reliably quantified by the recording of the electrocardiogram (ECG) in combination with the impedance cardiogram (ICG), which reflects the changes in thorax impedance in response to respiration and the ejection of blood from the ventricle into the aorta. From the respiration and ECG signals, respiratory sinus arrhythmia can be extracted as a measure of cardiac parasympathetic control. From the ECG and the left ventricular ejection signals, the preejection period can be extracted as a measure of cardiac sympathetic control. ECG and ICG recording is mostly done in laboratory settings. However, having the subjects report to a laboratory greatly reduces ecological validity, is not always doable in large scale epidemiological studies, and can be intimidating for young children. An ambulatory device for ECG and ICG simultaneously resolves these three problems.
Here, we present a study design for a minimally invasive and rapid assessment of cardiac autonomic control in children, using a validated ambulatory device 1-5, the VU University Ambulatory Monitoring System (VU-AMS, Amsterdam, the Netherlands,<a href="http://www.vu-ams.nl" target="_blank"> www.vu-ams.nl</a>).

Measuring Cardiac Autonomic Nervous System ANS Activity in Children

Measurement of autonomic nervous system activity usually confines the researcher and participant to the laboratory, which may provide an intimidating environment to children. The VU University Ambulatory Monitoring System (VU-AMS) device can record cardiac autonomic control in any setting. The VU-AMS proved very amenable to testing in children.

아이들의 측정 심장 자율 신경계 (ANS) 활동

The autonomic nervous system (ANS) controls mainly automatic bodily functions that are engaged in homeostasis, like heart rate, digestion, respiratory ...

Minimal Erythema Dose (MED) Testing

Ultraviolet radiation (UV) therapy is sometimes used as a treatment for various common skin conditions, including psoriasis, acne, and eczema. The dosage of UV light is prescribed according to an individual's skin sensitivity. Thus, to establish the proper dosage of UV light to administer to a patient, the patient is sometimes screened to determine a minimal erythema dose (MED), which is the amount of UV radiation that will produce minimal erythema (sunburn or redness caused by engorgement of capillaries) of an individual's skin within a few hours following exposure. This article describes how to conduct minimal erythema dose (MED) testing. There is currently no easy way to determine an appropriate UV dose for clinical or research purposes without conducting formal MED testing, requiring observation hours after testing, or informal trial and error testing with the risks of under- or over-dosing. However, some alternative methods are discussed.

Minimal Erythema Dose MED Testing

This article describes how to conduct minimal erythema dose (MED) testing in order to determine the lowest dose of ultraviolet radiation that will cause erythema (burning) when administered to an individual.

최소 홍반 량 (MED) 시험

Ultraviolet radiation (UV) therapy is  sometimes used as a treatment for various common skin conditions, including  psoriasis, acne, and eczema. The ...

A Novel Application of Musculoskeletal Ultrasound Imaging

Ultrasound is an attractive modality for imaging muscle and tendon motion during dynamic tasks and can provide a complementary methodological approach for biomechanical studies in a clinical or laboratory setting. Towards this goal, methods for quantification of muscle kinematics from ultrasound imagery are being developed based on image processing. The temporal resolution of these methods is typically not sufficient for highly dynamic tasks, such as drop-landing. We propose a new approach that utilizes a Doppler method for quantifying muscle kinematics. We have developed a novel vector tissue Doppler imaging (vTDI) technique that can be used to measure musculoskeletal contraction velocity, strain and strain rate with sub-millisecond temporal resolution during dynamic activities using ultrasound. The goal of this preliminary study was to investigate the repeatability and potential applicability of the vTDI technique in measuring musculoskeletal velocities during a drop-landing task, in healthy subjects. The vTDI measurements can be performed concurrently with other biomechanical techniques, such as 3D motion capture for joint kinematics and kinetics, electromyography for timing of muscle activation and force plates for ground reaction force. Integration of these complementary techniques could lead to a better understanding of dynamic muscle function and dysfunction underlying the pathogenesis and pathophysiology of musculoskeletal disorders.

We describe a new ultrasound-based vector tissue Doppler imaging technique to measure muscle contraction velocity, strain and strain rate with sub-millisecond temporal resolution during dynamic activities. This approach provides complementary measurements of dynamic muscle function and could lead to a better understanding of mechanisms underlying musculoskeletal disorders.

근골격계 초음파 이미징의 소설 응용 프로그램

Ultrasound is an attractive modality for imaging muscle and tendon  motion during dynamic tasks and can provide a complementary methodological  approach ...

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

MicroRNAs (miRNAs) constitute a potent layer of gene regulation by guiding RISC to target sites located on mRNAs and, consequently, by modulating their translational repression. Changes in miRNA expression have been shown to be involved in the development of all major complex diseases. Furthermore, recent findings showed that miRNAs can be secreted to the extracellular environment and enter the bloodstream and other body fluids where they can circulate with high stability. The function of such circulating miRNAs remains largely elusive, but systematic high throughput approaches, such as miRNA profiling arrays, have lead to the identification of miRNA signatures in several pathological conditions, including neurodegenerative disorders and several types of cancers. In this context, the identification of miRNA expression profile in the cerebrospinal fluid, as reported in our recent study, makes miRNAs attractive candidates for biomarker analysis.
There are several tools available for profiling microRNAs, such as microarrays, quantitative real-time PCR (qPCR), and deep sequencing. Here, we describe a sensitive method to profile microRNAs in cerebrospinal fluids by quantitative real-time PCR. We used the Exiqon microRNA ready-to-use PCR human panels I and II V2.R, which allows detection of 742 unique human microRNAs. We performed the arrays in triplicate runs and we processed and analyzed data using the GenEx Professional 5 software.
Using this protocol, we have successfully profiled microRNAs in various types of cell lines and primary cells, CSF, plasma, and formalin-fixed paraffin-embedded tissues.

We describe a protocol of real time PCR to profile microRNAs in the cerebrospinal fluid (CSF). With the exception of RNA extraction protocols, the procedure can be extended to RNA extracted from other body fluids, cultured cells, or tissue specimens.

정량 실시간 PCR을 사용하여 뇌척수액의 마이크로 RNA 프로파일

MicroRNAs (miRNAs) constitute a potent layer of gene regulation by guiding RISC to target sites located on mRNAs and, consequently, by modulating their ...

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of living systems. Neuromuscular diseases often exhibit a temporally varying, spatially heterogeneous, and multi-faceted pathology. The goal of this protocol is to characterize this pathology using qMRI methods. The MRI acquisition protocol begins with localizer images (used to locate the position of the body and tissue of interest within the MRI system), quality control measurements of relevant magnetic field distributions, and structural imaging for general anatomical characterization. The qMRI portion of the protocol includes measurements of the longitudinal and transverse relaxation time constants (T1 and T2, respectively). Also acquired are diffusion-tensor MRI data, in which water diffusivity is measured and used to infer pathological processes such as edema. Quantitative magnetization transfer imaging is used to characterize the relative tissue content of macromolecular and free water protons. Lastly, fat-water MRI methods are used to characterize fibro-adipose tissue replacement of muscle. In addition to describing the data acquisition and analysis procedures, this paper also discusses the potential problems associated with these methods, the analysis and interpretation of the data, MRI safety, and strategies for artifact reduction and protocol optimization.

Neuromuscular diseases often exhibit a temporally varying, spatially heterogeneous, and multi-faceted pathology. The goal of this protocol is to characterize this pathology using non-invasive magnetic resonance imaging methods.

골격근 질환의 정량적 자기 공명 영상

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of ...

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

The retinal pigment epithelium (RPE) is juxtaposed to the overlying sensory retina, and supports the function of the visual system. Among the tasks performed by the RPE are phagocytosis and processing of outer photoreceptor segments through lysosome-derived organelles. These degradation products, stored and referred to as lipofuscin granules, are composed partially of bisretinoids, which have broad fluorescence absorption and emission spectra that can be detected clinically as fundus autofluorescence with confocal scanning laser ophthalmoscopy (cSLO). Lipofuscin accumulation is associated with increasing age, but is also found in various patterns in both acquired and inherited degenerative diseases of the retina. Thus, studying its pattern of accumulation and correlating such patterns with changes in the overlying sensory retina are essential to understanding the pathophysiology and progression of retinal disease. Here, we describe a technique employed by our lab and others that uses cSLO in order to quantify the level of RPE lipofuscin in both healthy and diseased eyes.

The retinal pigment epithelium (RPE) supports the sensory retina through recycling visual cycle byproducts, which accumulate as lipofuscin. These products are autofluorescent and can be qualitatively imaged in vivo. Here, we describe a method to quantitatively image RPE lipofuscin using confocal scanning laser ophthalmoscopy.

망막 질환의 평가에 대한 정량적 안저 자기 형광

The retinal pigment epithelium (RPE) is juxtaposed to the overlying sensory retina, and supports the function of the visual system. Among the tasks ...

Measuring Cardiac Autonomic Nervous System (ANS) Activity in Toddlers - Resting and Developmental Challenges

The autonomic nervous system (ANS) consists of two branches, the parasympathetic and sympathetic nervous systems, and controls the function of internal organs (e.g., heart rate, respiration, digestion) and responds to everyday and adverse experiences 1. ANS measures in children have been found to be related to behavior problems, emotion regulation, and health 2-7. Therefore, understanding the factors that affect ANS development during early childhood is important. Both branches of the ANS affect young children&#8217;s cardiovascular responses to stimuli and have been measured noninvasively, via external monitoring equipment, using valid and reliable measures of physiological change 8-11. However, there are few studies of very young children with simultaneous measures of the parasympathetic and sympathetic nervous systems, which limits understanding of the integrated functioning of the two systems. In addition, the majority of existing studies of young children report on infants&#8217; resting ANS measures or their reactivity to commonly used mother-child interaction paradigms, and less is known about ANS reactivity to other challenging conditions. We present a study design and standardized protocol for a non-invasive and rapid assessment of cardiac autonomic control in 18 month old children. We describe methods for continuous monitoring of the parasympathetic and sympathetic branches of the ANS under resting and challenge conditions during a home or laboratory visit and provide descriptive findings from our sample of 140 ethnically diverse toddlers using validated equipment and scoring software. Results revealed that this protocol can produce a range of&#160;physiological responses to both resting and developmentally challenging conditions, as indicated by changes in heart rate and indices of parasympathetic and sympathetic activity. Individuals demonstrated&#160;variability in resting levels, responses to challenges, and challenge reactivity, which provides additional evidence that this protocol is useful for the examination of ANS individual differences for toddlers.

Measuring Cardiac Autonomic Nervous System ANS Activity in Toddlers - Resting and Developmental Challenges

We describe the methods for continuous monitoring of the autonomic nervous system under resting and challenge conditions with 18 month old children. Results revealed that this protocol can produce meaningful physiological responses in both branches of the autonomic nervous system and elicit significant individual variability in patterns of responses.

유아에서 심장 자율 신경계 (ANS) 활동을 측정 - 휴식 및 발달 과제

The autonomic nervous system (ANS) consists of two branches, the parasympathetic and sympathetic nervous systems, and controls the function of internal ...

Intracoronary Acetylcholine Provocation Testing for Assessment of Coronary Vasomotor Disorders

Intracoronary acetylcholine provocation testing (ACH-test) is an established method for assessment of epicardial coronary artery spasm in the catheterization laboratory which was introduced more than 30 years ago. Due to the short half-life of acetylcholine it can only be applied directly into the coronary arteries. Several studies have demonstrated the safety and clinical usefulness of this test. However, acetylcholine testing is only rarely applied in the U.S. or Europe. Nevertheless, it has been shown that 62% of Caucasian patients with stable angina and unobstructed coronary arteries on coronary angiography suffer from coronary vasomotor disorders that can be diagnosed with acetylcholine testing. In recent years it has been appreciated that the ACH-test not only assesses the presence of epicardial spasm but that it can also be useful for the detection of coronary microvascular spam. In such cases no epicardial spasm is seen after injection of acetylcholine but ischemic ECG shifts are present together with a reproduction of the patient's symptoms during the test. This article describes the experience with the ACH-test and its implementation in daily clinical routine.

Intracoronary acetylcholine testing has been established for the assessment of epicardial coronary spasm more than 30 years ago. Recently, the focus has shifted towards the microcirculation and it has been shown that microvascular spasm can be detected using ACH-testing. This article describes the ACH-test and its implementation in daily routine.

평가 관상 동맥의 혈관 운동 장애에 대한 관상 동맥 내 아세틸 콜린 도발 테스트

Intracoronary acetylcholine provocation testing (ACH-test) is an established method for assessment of epicardial coronary artery spasm in the ...

Proximal Cadaveric Femur Preparation for Fracture Strength Testing and Quantitative CT-based Finite Element Analysis

Cadaveric fracture testing is routinely used to understand factors that affect proximal femur strength. Because ex vivo biological tissues are prone to lose their mechanical properties over time, specimen preparation for experimental testing must be performed carefully to obtain reliable results that represent in vivo conditions. For that reason, we designed a protocol and a set of fixtures to prepare the femoral specimens such that their mechanical properties experienced minimal changes. The femora were kept in a frozen state except during preparation steps and mechanical testing. The relevant clinical measures of total hip and femoral neck bone mineral density (BMD) were obtained with a clinical dual X-ray absorptiometry (DXA) bone densitometer, and the 3D geometry and distribution of bone mineral were obtained using CT with a calibration phantom for quantitative estimations based on the greyscale values. Any possible bone disease, fracture, or the presence of implants or artifacts affecting the bone structure, was ruled out with X-ray scans. For preparation, all bones were carefully cleaned of excess soft tissue, and were cut and potted at the internal rotation angle of interest. A cutting fixture allowed the distal end of the bone to be cut off leaving the proximal femur at a desired length. To allow positioning of the femoral neck at prescribed angles during later CT scanning and mechanical testing, the proximal femoral shafts were potted in polymethylmethacrylate (PMMA) using a fixture designed specifically for desired orientations. The data collected from our experiments were then used for validation of quantitative computed tomography (QCT)-based finite element analysis (FEA), as described in a different protocol. In this manuscript, we present the protocol for the precise bone preparation for mechanical testing and subsequent QCT/FEA modeling. The current protocol was successfully applied to prepare about 200 cadaveric femora over a 6-year time period.

We present a robust protocol on how to carefully preserve and prepare cadaveric femora for fracture testing and quantitative computed tomography imaging. The method provides precise control over input conditions for the purpose of determining relationships between bone mineral density, fracture strength, and defining finite element model geometry and properties.

파괴 강도 시험 및 정량 CT 기반의 유한 요소 분석을위한 근위 대퇴골 사체 준비

Cadaveric fracture testing is routinely used to understand factors that affect proximal femur strength.  Because ex vivo biological tissues are prone to ...