The Third Affiliated Hospital of Guangzhou Medical University

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

Bangzhu Chen^1,2, Qinrong Lin^1,2, Nengqing Liu^1,2, Diyu Chen^1,2, Yinghui Zhang³, Xiaofang Sun^1,2

¹Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, ²Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, ³School of Biotechnology and Health Sciences, Wuyi University

Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.

Neuroscience

Ex Vivo Calcium Imaging for Drosophila Model of Epilepsy

Ming-Feng He¹, Chu-Qiao Liu², Xi-Xing Zhang², Yong-Miao Lin², Yu-Ling Mao^3,4, Jing-Da Qiao¹

¹Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital, Guangzhou Medical University, ²The Second Clinical Medicine School of Guangzhou Medical University, ³Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, ⁴Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University

Here, we present a protocol for ex vivo calcium imaging in GCaMP6-expressing adult Drosophila to monitor epileptiform activities. The protocol provides a valuable tool for investigating ictal events in adult Drosophila through ex vivo calcium imaging, allowing for exploration of the potential mechanisms of epilepsy at the cellular levels.

Medicine

Establishment and Validation of a Rat Model of Pulmonary Arterial Hypertension Associated with Pulmonary Fibrosis

Qian Jiang^*1, Dongmei Wen^*1, Fang Peng^*2, Wei Hong³, Xin Fu³, Yuanhui Xiong¹, Chongyuan Ren¹, Xuanyi Li¹, Dansha Zhou^1,4, Jian Wang^1,4, Yuqin Chen¹

¹State Key Laboratory of Respiratory Diseases, National Center for Respiratory Medicine, Guangdong Key Laboratory of Vascular Diseases, National Clinical Research Center for Respiratory Diseases, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, ²Department of Critical Care Medicine, The Third Affiliated Hospital of Guangzhou Medical University, ³GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, ⁴Guangzhou laboratory, Guangzhou International Bio Island

Here, the method of inducing pulmonary arterial hypertension associated with pulmonary fibrosis (PF-PH) rat model by injecting bleomycin into the airway is introduced. We also provide a step-by-step approach to validate this animal model.