JoVE Journal

Environment

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Nanyang Technological University

Lo screening per Endocrine attività in acqua mediante disponibili in commercio

A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.

Capitoli in questo video

0:05

Title

1:00

Prepare Dilutions of the Assay-specific Reference Chemical and Water Extract

2:42

Prepare the Cell Suspension to Conduct the Fret Bioassay

4:05

Plate Cells in a 96-well Black Wall Clear-bottom Plate and Add Diluted Water Extract

4:54

Measure Cytotoxicity and Endocrine Activity Response

5:51

Results

7:22

Conclusion

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