JoVE Journal

Biology

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Nanyang Technological University

Mapping the Cellular Distribution of an Optogenetic Protein Using a Light-Stimulation Grid

Transcript

This protocol involves transfecting cAMP sensors and bPAC-nLuc, an optogenetic protein, to accurately track its cellular distribution and response to light stimulation. The innovative approach of creating a cAMP response map using a point scanning system holds the potential for advancing research with optogenetic proteins in different fields.

Chapters in this video

0:00

Introduction

1:35

Light Stimulation and Live Cell Imaging to Study an Optogenetic Protein

8:05

Representative Results

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