JoVE Journal
Neuroscience
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Nanyang Technological University52.1K Views
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13:05 min
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December 18th, 2014
DOI :
December 18th, 2014
•0:05
Title
1:03
Neural Cell Harvesting
3:10
Antibody-independent Intracellular Labeling with Carboxyfluorescein Succinimidyl Ester (CFSE)
4:49
Cell-surface Antigen Staining with Fluorophore-conjugated Antibodies
5:56
Fixation and Membrane Permeabilization
7:35
Intracellular Antigen Staining with Non-fluorescent Primary Antibodies
9:30
Flow Cytometry Analysis
10:37
Results: Multicolor Flow Cytometric Analysis of Surface vs. Introcellular Antigens in Neural Cells
12:36
Conclusion
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ISSN 1940-087X
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