Desarrollo y validación de un método de PCR cuantitativa para Equid herpesvirus-2 Diagnóstico en los flujos respiratorios

Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.