RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

10.3K Views

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11:38 min

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November 26th, 2018

DOI :

10.3791/58687-v

November 26th, 2018

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Patrick S. McGrath¹^,²^,³, Nicole Diette¹^,², Igor Kogut¹^,²^,³, Ganna Bilousova¹^,²^,³

¹Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, ²Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, ³Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

Summary

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RNA based Reprogramming

Human Primary Fibroblasts

Induced Pluripotent Stem Cells

IPSCs

Transfection Efficiency

PH Adjustment

Sodium Hydroxide

Fibroblast Culture

Recombinant Human Laminin

Fibroblast Expansion Medium

Chapters in this video

0:04

Title

0:45

Reagents Preparation

2:01

Plating Fibroblasts for Reprogramming: Day 0

5:09

Initiation of Reprogramming: Day 1

9:28

Results: Efficient Reprogramming of Human Primary Fibroblasts into iPSCs

11:16

Conclusion

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