Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

13.0K Views

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08:42 min

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March 29th, 2018

DOI :

10.3791/57406-v

March 29th, 2018

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Tomoko Yamazaki¹^,², Wenling Li¹, Yoh-Suke Mukouyama¹

¹Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, ²Earle A. Chiles Research Institute, Robert W. Franz Cancer Center, Providence Portland Medical Center

Summary

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Keywords Whole mount

Confocal Microscopy

Ear Skin

Neuro vascular Branching

Morphogenesis

Immune Cell Distribution

Normal And Pathological Conditions

Chapters in this video

0:04

Title

1:03

Adult Mouse Skin Collection

2:50

Whole-mount Immunohistochemical Staining of Mouse Ear Skin

5:16

Mounting Ear Skin on Slide

6:42

Confocal Microscopy

7:13

Results: Neuro-vascular Branching Morphogenesis and Immune Cell Distribution in Adult Ear Skin

7:56

Conclusion

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