A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
8.8K Views
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08:55 min
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January 14th, 2020
DOI :
January 14th, 2020
•Sanat Upadhyay1, Leoncio Vergara2, Pranjali Shah2, Jan-Åke Gustafsson3,4, Ioannis Kakadiaris1,4, Maria Bondesson5
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics, University of Houston, 2Institute of Biosciences and Technology, Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, 4Department of Biosciences and Nutrition, Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering, Indiana University
Chapters in this video
0:04
Title
0:41
Fish Husbandry and Preparation of Solutions and Embryos
1:44
Mounting in Agarose and Optimization of Agarose Solution
3:38
Time-lapse Imaging
6:07
Results: Extended Time-lapse Imaging of Zebrafish Embryos
7:54
Conclusion
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