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Developmental Biology

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Nanyang Technological University

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

7.4K Views

•

05:48 min

•

January 2nd, 2018

DOI :

10.3791/56919-v

January 2nd, 2018

•

Ashutosh Pandey¹, Hamed Jafar-Nejad¹^,²

¹Department of Molecular and Human Genetics, Baylor College of Medicine, ²Program in Developmental Biology, Baylor College of Medicine

Summary

Complexity of in vivo systems makes it difficult to distinguish between the activation and inhibition of Notch receptor by trans- and cis-ligands, respectively. Here, we present a protocol based on in vitro cell-aggregation assays for qualitative and semi-quantitative evaluation of the binding of Drosophila Notch to trans-ligands vs cis-ligands.

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Cell Aggregation Assay

Aggregation Quantification

Chapters in this video

0:05

Title

0:38

Assessment of Binding Between Notch Receptor and Trans-ligands

1:47

Aggregation Between Signal-sending and Signal-receiving Cells

3:11

Evaluation of Inhibition of Binding Between Notch and Trans-ligands by Cis-ligands

4:15

Results: Faithful Analysis of Notch Signaling

5:30

Conclusion

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