Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

8.9K Views

•

08:41 min

•

December 4th, 2017

DOI :

10.3791/56419-v

December 4th, 2017

•

Matthias Becker¹^,², Janita A. Maring¹^,², Barbara Oberwallner², Benjamin Kappler², Oliver Klein¹^,², Volkmar Falk²^,³^,⁴^,⁵, Christof Stamm²^,³^,⁴

¹Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ²Berlin-Brandenburg Center for Regenerative Therapies (BCRT), ³German Center for Cardiovascular Research (DZHK), ⁴Deutsches Herzzentrum Berlin (DHZB), ⁵Department of Cardiovascular Surgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

Summary

Explore More Videos

Keywords Cardiac Extracellular Matrix

Hydrogel

Cell based Assays

Myocardial Infarction

Cardiac Tissue Engineering

Chapters in this video

0:05

Title

0:43

Sectioning Human Myocardium into Slices

2:27

Decellularization of Human Myocardium Slices

4:20

Processing the Decellularized cECM to a Powder

5:50

Enzyme-based Homogenization of cECM Micro-particles

6:56

Results: Effective Removal of Cellular Material Enhances Analysis

8:10

Conclusion

Related Videos

08:56

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

8.2K Views

07:34

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

9.0K Views

08:15

Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue

7.3K Views

14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

10.9K Views

06:10

Live Imaging of Mouse Secondary Palate Fusion

7.5K Views

09:51

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

15.2K Views

05:48

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

7.4K Views

14:55

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

10.1K Views

08:09

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

7.0K Views

09:48

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

3.9K Views