JoVE Journal

Developmental Biology

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Nanyang Technological University

Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

We describe a protocol for dissection, fixation, and immunostaining of steroidogenic organs in Drosophila larvae and adult females to study steroid hormone biosynthesis and its regulatory mechanism. In addition to steroidogenic organs, we visualize the innervation of steroidogenic organs as well as steroidogenic target cells such as germline stem cells.

Chapters in this video

0:05

Title

0:57

Dissecting the Larval Ring Gland-brain Complex

1:53

Filleting Larvae to Maintain Relative Tissue Positions

3:35

Fine Dissection of the Brain-ring Gland Complex and Mounting

5:34

Dissection of Adult Ovary

6:36

Dissection of Adult Reproductive Organs Along with Other Interactive Tissues

8:15

Mounting of Adult Structures and Imaging

9:33

Results: Preparation images

11:13

Conclusion

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