JoVE Journal

Developmental Biology

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Nanyang Technological University

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Here, a new method that allows the conversion of adult skin fibroblasts into insulin-secreting cells is presented. This technique is based on epigenetic conversion, does not involve the use of retroviral vectors nor the acquisition of a stable pluripotent state. It is therefore highly promising for translational medicine applications.

Chapters in this video

0:05

Title

0:50

Skin Fibroblast Isolation

1:53

Fibroblast Culture and Plating for Epigenetic Conversion

4:00

Increase Cell Plasticity Using the De-methylating Agent 5-aza-CR

5:31

Pancreatic Differentiation Protocol

6:47

Results: Morphological and Functional Changes During Epigenetic Conversion

8:02

Conclusion

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