JoVE Journal

Immunology and Infection

A subscription to JoVE is required to view this content.

على طريقة العائد فعالة وعالية لعزل مجموعات فرعية خلية الفأر الجذعية

Distinct dendritic cell subsets exist as rare populations in lymphoid organs, and therefore are challenging to isolate in sufficient numbers and purity for immunological experiments. Here we describe a high efficiency, high yield method for isolation of all of the currently known major subsets of mouse splenic dendritic cells.

Explore More Videos

Chapters in this video

0:05

Title

0:46

B16.Flt.3L Melanoma Harvest

1:40

Preparation of Splenic Single Cell Suspension from Tumor-bearing Mice

2:54

Plasmacytoid Dendritic Cell (DC) Isolation

4:35

CD8alpha+ and CD8alpha- DC Isolation

7:02

Results: Representative Splenic DC Subset Analysis from B16.Flt3L Tumor-bearing Mice

8:10

Conclusion

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More