This study has received funding from the Faculty of Dentistry, Mahidol University, Bangkok, Thailand. The authors thank Dr Erica Di Federico from School of Engineering and Materials Science and Dr Thomas Kelly from School of Geography at Queen Mary University of London for their expert technical inputs &#38; guidance in this work.

1. Tooth analog fabrication
<ol>
	<li>Anatomically reduce the occlusal surface of a typodont mandibular first molar (with bifurcated root) by 1 mm and bevel the margin using coarse and fine diamond burs.</li>
	<li>Scan the prepared typodont with a dental laboratory scanner.</li>
	<li>Open the scanned file with OrthoAnalyzer in the CAD software. On the Sculpt toolkit window, click on the Wax knife tool and set its diameter and level as 2.6 mm and 63&#160;&#956;m, respectively. Gradually pull each root surface toward each other to merge the bifurcated roots into a single root to facilitate the milling process (Figure 1).</li>
	<li>Mill the tooth analog dies from a high-pressure fiberglass laminate (n = 30) (Garolite, National Electrical Manufacturers Association [NEMA] grade G10) using a five-axis milling machine (Figure 2).</li>
</ol>
2. Mounting
<ol>
	<li>Use any appropriate CAD software (e.g., Autodesk Inventor Professional 2025) to design a jig that fits the root section of the model tooth and the space inside the PVC end caps to ensure standardized position and orientation of the test specimen.</li>
	<li>Print one jig per test toot in PMMA or similar modulus material using a 3D printer.</li>
	<li>Combine the root parts and dies with the PVC end cap, typically 25 mm in inner diameter, 21.5 mm high, and 5.5 mm in wall thickness.</li>
	<li>Mix the cold-curing low-viscosity epoxy resin and pour it up to the cementoenamel junction region of the model teeth. Be careful not to contaminate the occlusal surface of the model teeth with the flowing resin. Leave to fully set at room temperature for at least 24 h (Figure 3).</li>
</ol>
3. Occlusal veneer fabrication
<ol>
	<li>Import the scanned file of tooth analog to the CAD software.</li>
	<li>Under Directions, determine the insertion direction of the occlusal veneer.</li>
	<li>Under Interfaces, select Margin line and mark the margin line of the scanned tooth analog. Then, select Die interface | Advance settings and adjust the cement gap to 0.025 mm and the extra cement gap to 0.050 mm.</li>
	<li>Under Anatomy design, design a 1 mm-thick occlusal veneer using a template from Smile Library and adjust it with tools in Sculpt.</li>
	<li>Mill the lithium disilicate (IPS e.max CAD) and resin nanoceramic (Lava Ultimate) blocks (n=15 per group) using a five-axis milling machine and following the manufacturer&#39;s instructions (Figure 4).</li>
</ol>
4. Bonding and cementation
<ol>
	<li>Clean all master dies for 90 s in an&#160;ultrasonic machine and air dry. Then, apply 5% hydrofluoric acid to the occlusal surface for 60 s, thoroughly rinse with water, and air dry.</li>
	<li>For the LD group, etch the internal surface with 5% hydrofluoric acid for 20 s, thoroughly rinse with water, and air dry.</li>
	<li>For the RNC group, air-abrade with aluminum oxide powder grain size 50 &#181;m at 2 bars (200 kPa, 30 psi) for 10 s. Remove excess sand with alcohol and air dry.</li>
	<li>Apply silane, a universal bonding agent, and load dual-cured adhesive cement to the restoration intaglio. Place the restoration onto the prepared master dies by loading them under a silicone-filled compression head in an&#160;universal testing machine with a 40 N load.</li>
	<li>Cure with a light-emitting diode (LED) using light intensity in the normal mode of 1,000-1,200 mW/cm2 for 1-2 s. Remove excess cement, continue the curing light on each surface for 20 s, and leave it in the universal testing machine for 5 min.</li>
	<li>Remove from the universal testing machine and leave in distilled water at 37 &#176;C for 48 h to allow the cement to fully cure.</li>
	<li>Before testing, use fine permanent markers to draw three medial-lateral reference lines (sinistral, central, and dextral) and three anterior-posterior reference lines (upper, central, and lower) in different colors using fine permanent markers.</li>
</ol>
5. Quasistatic mechanical testing
<ol>
	<li>Position the test specimen in the center of the lower platen of a mechanical testing machine with a 5 kN load cell set up for compression testing.</li>
	<li>Place a 5.5 mm diameter stainless-steel ball in the central fossa of the restoration at the intersection of the central reference lines (Figure 5).</li>
	<li>Place a protective acrylic ring around the specimen and a debris shield in front of the testing machine to limit flying debris.</li>
	<li>Bring the crosshead down until nearly in contact with the steel ball and zero load and displacement.</li>
	<li>Apply compression at 1 mm/min until the restoration fractures, indicated by a sudden drop in the load. Record this load (Figure 5).</li>
	<li>After fracture, remove the shield and the acrylic ring, and carefully collect the test specimen and its fragments. The colored lines assist in placing the ceramic fragments into their original positions for subsequent analysis (Figure 5).</li>
	<li>Place the next test specimen and follow steps 5.2-5.6 until the groups are complete.</li>
</ol>
6. Statistical analysis
<ol>
	<li>Fill in Minitab worksheets with material codes and maximum loading values (N) retrieved from the experiment in the first and second columns, respectively.</li>
	<li>Conduct an independent t-test for two samples by selecting Stat | Basic Statistics | 2-sample t. Set the confidence level at 95% and assume equal variances.</li>
	<li>Create the Weibull distribution plot by selecting Stat | Reliability/Survival | Distribution Analysis (Right Censoring) | Distribution ID Plot. Choose maximum loading values in the Variable box, tick and choose material names in the By variable box, specify distribution as Weibull, and click OK.</li>
	<li>Conduct the Weibull statistical analysis by selecting Stat | Reliability/Survival | Distribution Analysis (Right Censoring) | Parametric Distribution Analysis | Weibull | Graphs. Choose the Probability plot | Display confidence intervals on above plots and click OK.</li>
</ol>
7. Fractographic analysis
<ol>
	<li>For stereo microscopy, mount the eyepiece camera and capture images (20x) of the samples&#39; aerial and side views via the stereomicroscopy software.</li>
	<li>For scanning electron microscopy, cut the specimen to the cementoenamel junction (CEJ), put it in an acetone bath in an ultrasonic cleaner&#160;and then air dry. Gold-coat and capture images (250-300 magnification) of the samples&#39; aerial and side views.</li>
</ol>

Quasistatic Load-to-Fracture Test of CAD/CAM Occlusal Veneers on Dentin Analogs

The authors have no conflicts of interest to declare.

In recent years, minimally invasive occlusal veneers have increasingly received attention in contemporary restorative dentistry. These restorations are usually fabricated from monolithic CAD/CAM glass-matrix ceramic, polycrystalline, and hybrid materials26. Conservative tooth preparation, the ease of access and visibility during tooth preparation, impression taking and cementation, and preservation of the marginal gingiva have been promoted as advantages26,<sup c...

Metal-free restorations are now highly preferred in anterior and posterior dentition due to their excellent optical characteristics and biocompatibility1. However, the major drawback of such materials is their susceptibility to fracture2. Most ceramics are vulnerable to cracks generated by tensile stresses, even under low strain3. Fractures of dental ceramic prostheses usually develop from slow radial crack growth due to long-term exposure to the tensile stresses generated during chewing4. Their weaknesses escalate with intrinsic flaws or defects within the materials and extrinsic flaws from fabrication and postprocessing5. Flexural strength, the capability to withstand tensile stress, of dental CAD/CAM materials can be achieved and compared through standard tests such as uniaxial (3-point or 4-point bending) and biaxial bending tests (ball-on-ring, ring-on-ring, and piston-on-three balls). Meanwhile, fracture toughness, the capability of a material to resist crack growth, can be derived from a single-edged notch beam and an indentation test. However, these tests cannot entirely predict and represent the behavior of the cemented prostheses with different anatomical configurations6. Other monotonic or dynamic mechanical tests have been introduced to justify their performance with various clinical aspects7,8.
A load to fracture or &#34;crunch the crown&#34; test has been used extensively in dentistry to investigate and compare the strengths of ceramic restorations with complex geometries9,10. Monotonic uniaxial compression is quasistatically exerted on the restorations in a vertical or lateral direction until a catastrophic fracture occurs. The fracture strength of the material can be determined from the maximum loading force, whereas the modes of fracture, including the site and direction of the crack(s), can be examined microscopically. A good restoration should be able to withstand both compressive and tensile stress from the voluntary maximum bite force, the highest masticatory force generated by jaw-elevator muscles under the influence of craniomandibular biomechanics and the reflex pathway11,12, which could be up to 900 N in the posterior teeth3. Furthermore, bruxism can involuntarily increase the force to 1,200 N in the same region13. In addition to material properties (i.e., elastic modulus), geometries, thicknesses, adhesive cement, and defect distributions influence any prosthesis&#39;s strength14. However, arguments have been raised on the clinical relevance of such tests due to the nonclinical high forces and the failure mechanisms being dissimilar to clinical situations6,14. A fatigue resistance test involving step-stress analysis and intraoral condition may be a more realistic approach for predicting the longevity of dental restorations7. Nevertheless, the load to fracture is still a quick, simple, and repeatable in vitro test to compare the strengths of new CAD/CAM ceramics materials launched onto the market where the manufacturer&#39;s data may not be dependable15,16,17. The result may reflect the prosthesis&#39;s tolerance to extreme forces from parafunction activities and unexpected clinical situations such as biting on hard seeds or gravels, which also causes failure in dental prostheses18,19,20,21.
With their increasing use to rehabilitate posterior teeth, the mechanical performance of occlusal veneers made from milled and printed CAD/CAM materials has been investigated for various aspects, including types of materials, prosthetic designs, tooth abutment preparation design, thicknesses, surface treatments, adhesive bonding, and luting cement system22,23. However, the data are still limited, and the test materials are from glass matrix ceramic and conventional CAD/CAM composite materials. An alternative hybrid material, resin nanoceramic, is now available. It claims to incorporate the strength of nanoceramic fillers and resiliency from resin matrix, which may be suitable for thin, minimally invasive restoration. However, its mechanical performance, especially in the molar region, requires more supporting evidence for clinical implications.
Until now, researchers have not had materials that can substitute for natural teeth in laboratory testing. High-pressure fiberglass laminate (National Electrical Manufacturers Association; NEMA grade G10) with the tradename of Garolite has been proposed as a dentin analog material for the mechanical testing of dental ceramics since 201014. It is a thermoset composite material comprising multilayers of fiberglass soaked in epoxy resin under high pressure. It can withstand high-stress conditions with similar elastic properties, fatigue behavior, and adhesive bond strengths as hydrated dentin14,24. It provides advantages over natural teeth regarding specimen preparation, standardization, and ethical authorization, with time savings due to reduced biosafety concerns24. Surface treatment can be performed by etching with 5% or 10% hydrofluoric acid from 60 s to 90 s and applying a silane coupling agent14,24. Nevertheless, studies on cemented prostheses with this material are limited, and the reliability of the existing evidence is still questionable24,25.
In this study, a laboratory protocol for a monotonic load-to-fracture test of 1 mm-thick occlusal veneers cemented to the master dies milled from dentin analog material against a&#160;nonfixed stainless steel ball was developed. The maximum load-bearing capacities of two dental CAD/CAM materials: lithium disilicate (LD) - IPS e.max CAD and resin nanoceramic (RNC) - Lava Ultimate, with n = 15 per group, were quantified and statistically compared through a two-sample independent t-test and Weibull statistical analysis. The fracture patterns were also investigated under optical stereomicroscopy and scanning electron microscopy. The study hypothesis was that this was an appropriate method of modeling the failure of occlusal veneers in clinical applications. The statistical null hypothesis was that there should be no difference in the maximum load-bearing capacities between the occlusal veneers made from the two materials.

<table><tbody><tr><td>3D printing (SLA)</td><td>&amp;nbsp;Formlabs, Somerville, MA, USA</td><td></td><td>Form3+</td></tr><tr><td>3Shape Dental Designer CAD software&amp;nbsp;</td><td>3Shape A/S, Copenhagen, Denmark</td><td></td><td>CAD software for tooth analog and veneers</td></tr><tr><td>5% hydrofluoric acid&amp;nbsp;</td><td>Ivoclar Vivadent, Schaan, Liechtenstein</td><td></td><td>IPS Ceramic Etching Gel</td></tr><tr><td>Alumina powder</td><td>Ronvig Dental Mfg. A/S, Daugaard, Denmark</td><td></td><td></td></tr><tr><td>Bluehill Universal materials testing software&amp;nbsp;</td><td>Instron Mechanical Testing Systems, Norwood, MA, USA</td><td></td><td></td></tr><tr><td>CamLabLite software&amp;nbsp;</td><td>Bresser UK Ltd, Kent, UK</td><td></td><td>Stereomicroscopy Software</td></tr><tr><td>Cold-curing low-viscosity epoxy resin&amp;nbsp;</td><td>Struers SAS, Champigny-sur-Marne, France</td><td></td><td></td></tr><tr><td>Dual-cure resin cement&amp;nbsp;</td><td>3M, Saint Paul, MN, USA</td><td></td><td>Rely X Ultimate Adhesive Resin Cement</td></tr><tr><td>Eyepiece camera&amp;nbsp;</td><td>ToupTek Photonics Co., Ltd., Hangzhou, China</td><td></td><td></td></tr><tr><td>High-pressure fibreglass laminate discs&amp;nbsp; (G10)</td><td>PAR Group Ltd, Lancashire, UK</td><td></td><td></td></tr><tr><td>IPS e.max CAD</td><td>Ivoclar Vivadent, Schaan, Liechtenstein</td><td>YB54G9/605330</td><td>Low translucency, A3, C14</td></tr><tr><td>Laboratory scanner&amp;nbsp;</td><td>3Shape A/S, Copenhagen, Denmark</td><td></td><td>D900L</td></tr><tr><td>Lava Ultimate</td><td>3M ESPE, Saint Paul, MN, USA</td><td>9541467/3314A3-LT</td><td>Low translucency, A3, 14L</td></tr><tr><td>Light-emitting diode (LED) curing light&amp;nbsp;</td><td>Woodpecker Medical Instrument, Guilin, China</td><td></td><td></td></tr><tr><td>Milling machine&amp;nbsp;</td><td>VHF camfacture AG, Amnnerbuch, Germany</td><td></td><td>VHF S2</td></tr><tr><td>Minitab 18&amp;nbsp;</td><td>Minitab Inc, State College, PA, USA</td><td></td><td></td></tr><tr><td>nQuery Advisor Version 9.2.10&amp;nbsp;</td><td>Statistical Solutions Ltd., CA, USA</td><td></td><td>Statistical Software</td></tr><tr><td>Polyvinyl chloride end cap&amp;nbsp;</td><td>Plastic Pipe Shop Ltd, Stirling, UK</td><td></td><td>25 mm X 21.5 mm;</td></tr><tr><td>Scanning electron microscope&amp;nbsp;</td><td>Tescan, Brno, Czech Republic</td><td></td><td>Tescan Vega</td></tr><tr><td>Silane coupling agent&amp;nbsp;</td><td>3M, Saint Paul, MN, USA</td><td></td><td>RelyX Ceramic Primer</td></tr><tr><td>Autodesk Inventor Professional 2024</td><td>Autodesk, San Francisco, CA, USA</td><td></td><td>CAD software for jig</td></tr><tr><td>Sputter vacuum coater&amp;nbsp;&amp;nbsp;</td><td>Quorum, East Sussex, UK</td><td></td><td>MiniQS Sputter Coater</td></tr><tr><td>Stata18&amp;nbsp;</td><td>StataCorp LLC, College Station, TX, USA</td><td></td><td></td></tr><tr><td>Stereomicroscope&amp;nbsp;</td><td>Carl Zeiss AG, Oberkoche, Germany</td><td></td><td>Zeiss Stemi 508</td></tr><tr><td>Typodont mandibular first molar&amp;nbsp;</td><td>Frasaco GmbH, Tettnang, Germany</td><td></td><td>ANA-4 Z3RN-36</td></tr><tr><td>Universal dental bonding agent&amp;nbsp;</td><td>&amp;nbsp;3M, Saint Paul, MN, USA</td><td></td><td>Scotch Bond Universal Adhesive</td></tr><tr><td>Universal testing machine</td><td>Instron Mechanical Testing Systems, Norwood, MA, USA</td><td></td><td>Intron 5900-84&amp;nbsp;</td></tr></tbody></table>

quasistatic mechanical testing for computer-aided design and manufacturing occlusal veneers cemented to milled dentin analog material

Under current minimally invasive treatment regimes, minor tooth preparation and thinner biomimetic ceramic restoration are used to preserve the restored tooth&#39;s vitality, aesthetics, and function. New computer-aided design and computer-aided manufacturing (CAD/CAM) ceramic-like material are&#160;now available. To guarantee longevity, a dental clinician must know these&#160;newly launched product&#39;s mechanical strength compared to the relatively brittle glass-matrix ceramic. Furthermore, a tooth substitute has been promoted for laboratory investigation, especially after the pandemic, and more evidentiary support is required for its application.
This study developed a laboratory protocol for a monotonic load-to-fracture test to determine the fracture strength of 1 mm-thick CAD/CAM occlusal veneers. Master dies were milled from high-pressure fiberglass laminate, which has&#160;similar elastic modulus and bond strength as hydrated dentin. They were mounted into polyvinyl chloride (PVC) end caps with cold-curing epoxy resin. Occlusal veneers, also called tabletop restorations, were milled from lithium disilicate (LD) and resin nanoceramic blocks (RNC) and cemented to prepared master dies using dual-cured adhesive resin cement. They were&#160;allowed to cure fully by storing in distilled water for 48 h at 37 &#176;C.
All samples were then placed in a universal testing machine and loaded via a non-fixed 5.5 mm stainless-steel ball that allows lateral movement as would occur against the antagonist&#160;teeth. Compression was applied at a 1 mm/min rate, and the load-displacement graph was generated. The average maximum load-bearing capacity of restorations in the RNC group (3,212.80 &#177; 558.67 N) was significantly higher than in the LD group (2727.10 &#177; 472.41 N) (p &#60; 0.05). No debonding was found during the test.&#160;Both CAD/CAM materials may have a similar flaw distribution. Hertzian cone crack was found at the loading site, whereas radial cracks propagating from the cementation surface were found close to the margin in both groups.

The sample size calculation was performed using the referenced software, which generated an effect size of 0.39 and suggested a minimum sample size of n = 13 per group. However, a sample size of n = 15 was chosen in this study to detect the difference of 5%. The null hypothesis was rejected. Despite having greater flexural strength, the mean values of the maximum loading force of the 1 mm-thick occlusal veneers (n = 15 per group) made from the LD (lithium disilicate: 2,727.10 &#177; 472.41 N) group were significantly low...

Quasistatic Mechanical Testing for Computer-Aided Design and Manufacturing Occlusal Veneers Cemented to Milled Dentin Analog Material

A quasistatic load-to-fracture test with a non-fixed stainless steel ball was developed to determine the fracture strength of minimally invasive posterior computer-aided design and manufacturing restorations cemented to dentin analog materials. This test models the typical loading regime responsible for the fracture of dental restorations.

Under current minimally invasive treatment regimes, minor tooth preparation and thinner biomimetic ceramic restoration are used to preserve the restored ...

quasistatic-mechanical-testing-for-computer-aided-design

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Research

JoVE Journal

Medicine

270 Views.  Queen Mary University of London. A quasistatic load-to-fracture test with a non-fixed stainless steel ball was developed to determine the fracture strength of minimally invasive posterior computer-aided design and manufacturing restorations cemented to dentin analog materials. This test models the typical loading regime responsible for the fracture of dental restorations.

Video: Quasistatic Mechanical Testing for Computer-Aided Design and Manufacturing Occlusal Veneers Cemented to Milled Dentin Analog Material

In situ Compressive Loading and Correlative Noninvasive Imaging of the Bone-periodontal Ligament-tooth Fibrous Joint

This study demonstrates a novel biomechanics testing protocol. The advantage of this protocol includes the use of an in situ loading device coupled to a high resolution X-ray microscope, thus enabling visualization of internal structural elements under simulated physiological loads and wet conditions. Experimental specimens will include intact bone-periodontal ligament (PDL)-tooth fibrous joints. Results will illustrate three important features of the protocol as they can be applied to organ level biomechanics: 1) reactionary force vs. displacement: tooth displacement within the alveolar socket and its reactionary response to loading, 2) three-dimensional (3D) spatial configuration and morphometrics: geometric relationship of the tooth with the alveolar socket, and 3) changes in readouts 1 and 2 due to a change in loading axis, i.e. from concentric to eccentric loads. Efficacy of the proposed protocol will be evaluated by coupling mechanical testing readouts to 3D morphometrics and overall biomechanics of the joint. In addition, this technique will emphasize on the need to equilibrate experimental conditions, specifically reactionary loads prior to acquiring tomograms of fibrous joints. It should be noted that the proposed protocol is limited to testing specimens under ex vivo conditions, and that use of contrast agents to visualize soft tissue mechanical response could lead to erroneous conclusions about tissue and organ-level biomechanics.

In situ Compressive Loading and Correlative Noninvasive Imaging of the Bone-periodontal Ligament-tooth Fibrous Joint

In this study, the use of an in situ loading device coupled with micro-X-ray computed tomography for fibrous joint biomechanics will be discussed. Experimental readouts identifiable with an overall change in joint biomechanics will include: 1) reactionary force vs. displacement, i.e. tooth displacement within the alveolar socket and its reactionary response to loading, 2) three-dimensional (3D) spatial configuration and morphometrics, i.e.&#160;geometric relationship of the tooth with the alveolar socket, and 3) changes in readouts 1 and 2 due to a change in loading axis, i.e. concentric or eccentric loads.

This study demonstrates a novel biomechanics testing protocol. The advantage of this protocol includes the use of an in situ loading device coupled to a ...

Bioengineering

An Improved Mechanical Testing Method to Assess Bone-implant Anchorage

Recent advances in material science have led to a substantial increase in the topographical complexity of implant surfaces, both on a micro- and a nano-scale. As such, traditional methods of describing implant surfaces -&#160;namely numerical determinants of surface roughness -&#160;are inadequate for predicting in vivo performance. Biomechanical testing provides an accurate and comparative platform to analyze the performance of biomaterial surfaces. An improved mechanical testing method to test the anchorage of bone to candidate implant surfaces is presented. The method is applicable to both early and later stages of healing and can be employed for any range of chemically or mechanically modified surfaces -&#160;but not smooth surfaces. Custom rectangular implants are placed bilaterally in the distal femora of male Wistar rats and collected with the surrounding bone. Test specimens are prepared and potted using a novel breakaway mold and the disruption test is conducted using a mechanical testing machine. This method allows for alignment of the disruption force exactly perpendicular, or parallel, to the plane of the implant surface, and provides an accurate and reproducible means for isolating an exact peri-implant region for testing.

An improved method to mechanically test bone anchorage to candidate implant surfaces is presented. This method allows for alignment of the disruption force exactly perpendicular, or parallel, to the plane of the implant surface, and provides an accurate means to direct the disruption forces to an exact peri-implant region.

Recent advances in material science have led to a substantial increase in the topographical complexity of implant surfaces, both on a micro- and a ...

Shrinkage of Dental Composite in Simulated Cavity Measured with Digital Image Correlation

Polymerization shrinkage of dental resin composites can lead to restoration debonding or cracked tooth tissues in composite-restored teeth. In order to understand where and how shrinkage strain and stress develop in such restored teeth, Digital Image Correlation (DIC) was used to provide a comprehensive view of the displacement and strain distributions within model restorations that had undergone polymerization shrinkage.
Specimens with model cavities were made of cylindrical glass rods with both diameter and length being 10 mm. The dimensions of the mesial-occlusal-distal (MOD) cavity prepared in each specimen measured 3 mm and 2 mm in width and depth, respectively. After filling the cavity with resin composite, the surface under observation was sprayed with first a thin layer of white paint and then fine black charcoal powder to create high-contrast speckles. Pictures of that surface were then taken before curing and 5 min after. Finally, the two pictures were correlated using DIC software to calculate the displacement and strain distributions.
The resin composite shrunk vertically towards the bottom of the cavity, with the top center portion of the restoration having the largest downward displacement. At the same time, it shrunk horizontally towards its vertical midline. Shrinkage of the composite stretched the material in the vicinity of the &#8220;tooth-restoration&#8221; interface, resulting in cuspal deflections and high tensile strains around the restoration. Material close to the cavity walls or floor had direct strains mostly in the directions perpendicular to the interfaces. Summation of the two direct strain components showed a relatively uniform distribution around the restoration and its magnitude equaled approximately to the volumetric shrinkage strain of the material.

In order to understand the spatial development of polymerization shrinkage stress in dental resin-composite restorations, Digital Image Correlation was used to provide full-field displacement/strain measurement of restored model glass cavities by correlating images of the restoration taken before and after polymerization.

Polymerization shrinkage of dental resin composites can lead to restoration debonding or cracked tooth tissues in composite-restored teeth. In order to ...

Roughness Impact of Piezoelectric Dental Scaler on Two Distinct Flowable Composite Filling Materials

Dental ultrasonic scalers are commonly employed in periodontal treatment; however, their ability to roughen tooth surfaces is a worry since roughness may increase plaque production, a key cause of periodontal disease. This research studied the influence of a piezoelectric ultrasonic scaler on the roughness of two distinct flowable composite filling materials. To do this, 10 disc-shaped samples were generated from each of the two flowable composite materials. After standardized polishing, samples were submerged in water for 24 h before the first surface examination using electron microscopy and profilometry. The ultrasonic scaler was applied to a specified location of each sample for 60 s under water cooling and regulated force. Post-scaler surface parameters were again examined. Following the application of the scaler, both composite materials exhibited a notable increase in surface roughness, as determined by profilometry (p &lt; 0.01). Additionally, the observed surface roughness was also qualitatively visualized with scanning electron microscopy. While initial roughness levels were comparable across the two composites (p = 0.143) after scaler application, no substantial discrepancy in surface texture was noticed between them (p = 0.684). The use of a high-power piezoelectric ultrasonic scaler on routinely used flowable composite restorations might generate considerable surface roughness, possibly leading to increased plaque accumulation. Nevertheless, it might be postulated that nanohybrid flowable composite materials having conventional monomer ingredients may demonstrate comparable surface alterations within the limitations of this experiment.

This methodology allows applying a dental appliance on any specimen at any angle with standardized force and stability. This approach might be extensively used in health sciences to standardize the impacts of dental equipment with hand-holding elements such as micromotors, turbines, and ultrasonic scalers on varied surfaces.

Dental ultrasonic scalers are commonly employed in periodontal treatment; however, their ability to roughen tooth surfaces is a worry since roughness may ...

Behavior

Mimicking and Measuring Occlusal Erosive Tooth Wear with the "Rub&amp;Roll" and Non-contact Profilometry

Chewing, drinking, and occasional tooth grinding will result in physiological tooth wear during a lifetime. Extreme challenges, such as bruxism or habitual chewing on foreign objects, may lead to excessive wear. Recently, the role of erosion in accelerating mechanical tooth wear has been recognized, but the interplay between chemical and mechanical wear processes has not been extensively studied. Our laboratory recently introduced a novel oral wear simulation device, the Rub&#38;Roll, that enables the user to perform wear and loading studies separately or simultaneously in an erosive and/or abrasive environment. This manuscript describes an application of the device: the combined mechanical and erosive loading of extracted human (pre)molars in a simulated chewing movement, with a controlled application of force, velocity, fluid, and time, and the application of non-contact profilometry in visualizing and measuring the resulting wear pattern. The occlusal morphology that was created in the experiment with the highest loading level is very similar to the clinical presentation of erosive wear.

Mimicking and Measuring Occlusal Erosive Tooth Wear with the "Rub&Roll" and Non-contact Profilometry

The Rub&#38;Roll can mimic the chewing cycle, allowing variation of chewing force, sliding distance, chewing velocity, number of cycles, and frequency, and with a combination of erosive and abrasive challenges can result in a complex simulation of oral ageing.

Chewing, drinking, and occasional tooth grinding will result in physiological tooth wear during a lifetime. Extreme challenges, such as bruxism or ...

Force System with Vertical V-Bends: A 3D In Vitro Assessment of Elastic and Rigid Rectangular Archwires

A proper understanding of the force system created by various orthodontic appliances can make treatment of patients efficient and predictable. Reducing the complicated multi-bracket appliances to a simple two-bracket system for the purpose of force system evaluation will be the first step in this direction. However, much of the orthodontic biomechanics in this regard is confined to 2D experimental studies, computer modeling/analysis or theoretical extrapolation of existing models. The objective of this protocol is to design, construct and validate an in vitro 3D model capable of measuring the forces and moments generated by an archwire with a V-bend placed between two brackets. Additional objectives are to compare the force system generated by different types of archwires among themselves and to previous models. For this purpose, a 2 x 4 appliance representing a molar and an incisor has been simulated. An orthodontic wire tester (OWT) is constructed consisting of two multi-axis force transducers or load cells (nanosensors) to which the orthodontic brackets are attached. The load cells are capable of measuring the force system in all the three planes of space. Two types of archwires, stainless-steel and beta-titanium of three different sizes (0.016 x 0.022 inch, 0.017 x 0.025 inch and 0.019 x 0.025 inch), are tested. Each wire receives a single vertical V-bend systematically placed at a specific position with a predefined angle. Similar V-bends are replicated on different archwires at 11 different locations between the molar and incisor attachments. This is the first time an attempt has been made in vitro to simulate an orthodontic appliance utilizing V-bends on different archwires.

Force System with Vertical V-Bends: A 3D In Vitro Assessment of Elastic and Rigid Rectangular Archwires

The method presented here is designed to construct and validate an in vitro 3D model capable of measuring the force system generated by different archwires with V-bends placed between two brackets. Additional objectives are to compare this force system with different types of archwires and to previous models.

A proper understanding of the force system created by various orthodontic appliances can make treatment of patients efficient and predictable. Reducing ...

Precision of In Vivo Quantitative Tooth Wear Measurement Using Intra-Oral Scans

Quantitative wear measurement is of increasing interest for measuring tooth wear progression. However, most research on quantitative wear measurement has focused on simulated wear or scanned gypsum casts. A 3D Wear Analysis (3DWA) protocol has been developed that analyzes tooth wear in vivo through intra-oral scanners available to dental clinicians. This study investigated the precision of the 3DWA protocol for measuring wear through maximum height loss (mm) and volume change (mm3). Observational prospective wear data from 55 patients were analyzed after 0-1-, 0-3-, and 0-5-year intervals to determine rates of wear, and convenience samples were chosen to test the protocol&#39;s precision on dentitions scanned twice in one sitting and its intra- and inter-rater precision on scans with 0-3- and 0-5-year intervals. Scans were made using intra-oral scanners (IOS) and superimposed using 3D measurement software. T-tests were performed to determine the structural and random error, and trimmed ranges were calculated to interpret the error. For protocol precision, the mean difference was 0.015 mm (-0.002; 0.032, p = 0.076) for height and -0.111 mm3 (-0.250; 0.023, p = 0.101) for volume. The duplicate measurement error was 0.062 mm for height and 0.268 mm3 for volume. The height measurements were precise enough to measure wear after intervals of 0-3 or 0-5 years; however, volume measurements were susceptible to procedural error and operator sensitivity. The 3DWA protocol is precise enough to adequately measure tooth height loss after intervals of a minimum of 3 years or in patients with severe wear progression, but it is not suited to measuring volumetric changes.

Precision of In Vivo Quantitative Tooth Wear Measurement Using Intra-Oral Scans

Quantitative wear measurement is a method of increasing importance in measuring tooth wear progression. We here describe a protocol, its precision, and its intra/inter-rater precision for the acquisition and superimposition of repeated in vivo scanned dentitions in patients with moderate to severe wear, reporting on both height and volume measurements.

Quantitative wear measurement is of increasing interest for measuring tooth wear progression. However, most research on quantitative wear measurement has ...

Evaluation of Maxillary Posterior Tooth Movement Using Digital Superimposition

Measuring Maxillary Posterior Tooth Movement: A Model Assessment using Palatal and Dental Superimposition

Since the introduction of Invisalign by Align Technology, Inc. in 1999, questions and debates have persisted regarding the precision of Invisalign (clear aligner) therapy, particularly when compared to the use of traditional fixed appliances. This becomes particularly significant in cases involving anteroposterior, vertical, and transverse corrections, where precise comparisons are of paramount importance. To address these inquiries, this study introduces a meticulously devised protocol, placing a primary emphasis on digitally superimposing the movement of maxillary posterior teeth to facilitate accurate analysis. The sample included 25 patients who had completed their first series of Invisalign (clear) aligners. Four maxillary digital models (pre-treatment, post-treatment, ClinCheck-initial, and final models) were digitally superimposed using the palate rugae and dentitions as stable references. A software combination was used for model superimposition and tooth segmentation. Transformation matrices then expressed the differences between the achieved and predicted tooth positions. Thresholds for clinically relevant differences were at &plusmn;0.25 mm for linear displacement and &plusmn;2&#176; for rotation. Differences were assessed using Hotelling's T-squared tests with Bonferroni correction. The mean differences in rotation (2.036&#176; &plusmn; 4.217&#176;) and torque (-2.913&#176; &plusmn; 3.263&#176;) were significant statistically and clinically, with p-values of 0.023 and 0.0003 respectively. De-rotation of premolars and torque control for all posterior teeth were less predictable. All mean differences for the linear measurements were statistically and clinically insignificant, except that the first molars seemed slightly (0.256 mm) more intruded than their predicted position. The clear aligner system appears to meet its prediction for most translational tooth movements and mesial-distal tipping in maxillary posterior teeth for non-extraction cases with mild to moderate malocclusions.

Author Spotlight: 3D Movement Assessment of Maxillary Posterior Teeth in Clear Aligner Treatment

This manuscript presents a comprehensive protocol to evaluate the three-dimensional (3D) movement of maxillary posterior teeth with clear aligners using digital model superimposition, an invaluable tool in orthodontics and dentofacial orthopedics.

Since the introduction of Invisalign by Align Technology, Inc. in 1999, questions and debates have persisted regarding the precision of Invisalign (clear ...

Impact of Fabrication Techniques and Polishing Procedures on Surface Roughness of Denture Base Resins

This study aimed to assess the impact of various fabrication techniques and polishing procedures on the surface roughness (Ra) of resin-based materials used in the fabrication of complete dentures. A total of 90 specimens were produced from three different resin materials: heat-polymerized polymethyl methacrylate (PMMA) resin, CAD-CAM milled PMMA resin, and 3D-printed resin (n = 30). Each specimen measured 10 mm in diameter and 2 mm in height. The surface roughness (Ra) values of the specimens were initially determined using a contact profilometer following fabrication. Subsequently, each group of specimens was polished with 600-, 800-, and 1000-grit silicon carbide abrasive papers under running water. A second measurement of the surface roughness (Ra) values was then performed. The data were analyzed statistically using the Kruskal-Wallis test, Mann-Whitney U test, Wilcoxon signed-rank test, and paired samples t-test (p = 0.05). A statistically significant difference was identified between the groups in terms of surface roughness (Ra) prior to the polishing process (p &#60; 0.001). However, no statistically significant difference was observed between the milled and heat-polymerized PMMA base materials following the polishing process. The 3D-printed specimens showed the most notable improvement in surface roughness due to the polishing process. Nevertheless, their surface roughness remained statistically significantly higher compared to the other samples, both before and after polishing (p &#60; 0.001). The fabrication method of complete denture base materials was observed to influence surface roughness. The surface roughness values of the base materials fabricated using the 3D printing method were higher compared to those fabricated with milled and heat-polymerized PMMA resin, both before and after polishing.

This article presents a protocol to evaluate the effects of different manufacturing methods (heat-polymerized PMMA, CAD-CAM milled PMMA, and 3D-printed resin) and polishing techniques (600, 800, and 1000 grit silicon carbide abrasive papers) on the surface roughness (Ra) of resin base materials used for complete dentures.

This study aimed to assess the impact of various fabrication techniques and polishing procedures on the surface roughness (Ra) of resin-based materials ...

Microhardness Protocol for Evaluating Rodent Oral Disease

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models

The mechanical property, microhardness, is evaluated in dental enamel, dentin, and bone in oral disease models, including dental fluorosis and periodontitis. Micro-CT (&#181;CT) provides 3D imaging information (volume and mineral density) and scanning electron microscopy (SEM) produces microstructure images (enamel prism and bone lacuna-canalicular). Complementarily to structural analysis by &#181;CT and SEM, microhardness is one of the informative parameters to evaluate how structural changes alter mechanical properties. Despite being a useful parameter, studies on microhardness of alveolar bone in oral diseases are limited. To date, divergent microhardness measurement methods have been reported. Since microhardness values vary depending on the sample preparation (polishing and flat surface) and indentation sites, diverse protocols can cause discrepancies among studies. Standardization of the microhardness protocol is essential for consistent and accurate evaluation in oral disease models. In the present study, we demonstrate a standardized protocol for microhardness analysis in tooth and alveolar bone. Specimens used are as follows: for the dental fluorosis model, incisors were collected from mice treated with/without fluoride-containing water for 6 weeks; for ligature-induced periodontal bone resorption (L-PBR) model, alveolar bones with periodontal bone resorption were collected from mice ligated on the maxillary 2nd molar. At 2 weeks after the ligation, the maxilla was collected. Vickers hardness was analyzed in these specimens according to the standardized protocol. The protocol provides detailed materials and methods for resin embedding, serial polishing, and indentation sites for incisors and alveolar. To the best of our knowledge, this is the first standardized microhardness protocol to evaluate the mechanical properties of tooth and alveolar bone in rodent oral disease models.

Author Spotlight: Establishing an Accurate Microhardness Testing Protocol for Craniofacial Tissues

Microhardness is a mechanical property and an informative parameter for evaluating hard tissue pathophysiology. Here, we demonstrate a standardized protocol (sample preparation, polishing, flat surface, and indentation sites) for microhardness analysis in tooth and alveolar bone in rodent oral disease models, namely, dental fluorosis, and ligature-induced periodontal bone resorption.

Quasistatic Mechanical Testing for Computer-Aided Design and Manufacturing Occlusal Veneers Cemented to Milled Dentin Analog Material

Summary

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Precision of In Vivo Quantitative Tooth Wear Measurement Using Intra-Oral Scans

Measuring Maxillary Posterior Tooth Movement: A Model Assessment using Palatal and Dental Superimposition

Impact of Fabrication Techniques and Polishing Procedures on Surface Roughness of Denture Base Resins

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models