Generation and Functional Verification of Hypoxia-Sensitive Chimeric Antigen Receptor-T Cells

692 Views

•

09:12 min

•

June 14th, 2024

DOI :

10.3791/66697-v

June 14th, 2024

•

Ying Xue*¹, Yunyu Mao*², Qibin Liao³, Chen Zhao⁴, Xiaoyan Zhang¹^,²^,⁴, Jianqing Xu¹^,²^,⁴

¹Institutes of Biomedical Sciences, Fudan University, ²Clinical Center of Biotherapy at Zhongshan Hospital, Fudan University, ³Department of Oncology and Bio-therapeutic Center, Shenzhen Third People's Hospital, Southern University of Science and Technology, ⁴Shanghai Public Health Clinical Center, Fudan University

Summary

Explore More Videos

Hypoxia sensitive CAR T cells

Cancer Immunotherapy

CAR T cell Therapy

Tumor Microenvironment

Tumor Selectivity

Chimeric Antigen Receptor

Hypoxia conditioned CAR

Safety Profile

Efficacy Enhancement

Functional Verification

Oxygen dependent Degradation Domain

Cancer Treatment Protocol

Cytotoxicity Analysis

Mobile Incubator Chamber

Chapters in this video

0:00

Introduction

1:43

Development of Hypoxia‐Responsive CAR‐T Cells via Lentiviral Transduction

4:32

Flow Cytometric Assessment of Oxygen‐Dependent CAR Expression in CAR‐T cells

7:14

In Vitro Assessment of Cytotoxicity Mediated by Hypoxia‐Sensitive CAR‐T Cells

Related Videos

05:22

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

10.3K Views

08:04

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

11.5K Views

06:51

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

14.8K Views

10:57

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

10.3K Views

07:03

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

4.2K Views

07:47

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

4.0K Views

09:34

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [¹⁸F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

3.2K Views

09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

4.8K Views

05:49

Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection

2.8K Views

06:08

Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

1.2K Views