Ex Vivo Culture of Circulating Tumor Cells in the Cerebral Spinal Fluid from Melanoma Patients to Study Melanoma-Associated Leptomeningeal Disease
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06:25 min
•
March 29th, 2024
DOI :
March 29th, 2024
•Vincent Law1,2, Inna Smalley3, Brittany R. Evernden2, Margaret Baldwin4, Keiran S. M. Smalley1, Peter A. Forsyth1,2
1Department of Tumor Biology, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute, 2Department of Neuro-Oncology, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute, 3Department of Cancer Physiology, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute, 4Department of Comparative Medicine, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute
Chapters in this video
0:00
Introduction
1:36
Preparation of Human Meningeal Cell (HMC) Conditioned Media and Collection of Cerebrospinal Fluid for the Culture of Circulating Tumor Cells
3:23
In Vitro Culture and Expansion of Cerebro Spinal Fluid (CSF)-Circulating Tumor Cells (CTCs) for the Study of Leptomeningeal Disease
4:24
CSF Collection from Mice with Leptomeningeal Disease for Subsequent Clone Expansion
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