Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
2.4K Views
•
13:19 min
•
April 26th, 2024
DOI :
April 26th, 2024
•Chapters in this video
0:12
Introduction
1:07
Setting up the JIMT-1 Enhanced Fluorescent Protein (EGFP) Spheroid Model
3:43
Coating the HCS Plate
4:33
Transfer of Spheroids to the HCS Plate
4:46
Pre-treatment of JIMT-1-EGFP Spheroids with Test Compounds
5:14
Induction of Antibody-Dependent Cellular Cytotoxicity (ADCC) by Adding the Effector Cells
7:10
Annexin V-647 Staining of Spheroids
7:34
Imaging
11:43
Representative Results
12:52
Conclusion
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