Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

3.4K Views

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05:44 min

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November 25th, 2022

DOI :

10.3791/64776-v

November 25th, 2022

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Ying Wang¹^,², Zhuo An¹^,², Zhifang Zhao¹^,², Can Li³, Jiangbo Fan¹^,²

¹Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, ²Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, ³Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University

Summary

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Reactive Oxygen Species

Immune Response

Rice

Chemiluminescence Assay

Murashige And Skoog Medium

Horse Radish Peroxidase

Flg22

Experimental Protocol

Chapters in this video

0:05

Introduction

0:36

Plant Culture

1:20

Tissue Preparation and Pretreatment

2:37

Setting up the Protocol with the Microplate Reader, Establishing the Elicitation System, and Measuring Real-Time ROS Production

3:42

Results: ROS Induction, the Total Amount of ROS Generated, and ROS Production in the Exposed Cells of the Cut Edge

5:07

Conclusion

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