Long-Term Culture and Monitoring of Isolated Caenorhabditis elegans on Solid Media in Multi-Well Devices
1.6K Views
•
09:32 min
•
December 9th, 2022
DOI :
December 9th, 2022
•Chapters in this video
0:05
Introduction
1:03
Preparation of the Multi‐Well Device
3:00
Preparation of the Multi‐Well Device for Media Loading
3:29
Filling the Wells with Low Melting Nematode Growth Media (lmNGM)
5:53
Adding Copper Sulfate to the Moat and Adding Autoclaved Water Crystals
6:55
Spotting the Wells with Concentrated Bacteria
7:29
Adding Worms to the Multi‐Well Device and Finishing the Preparation of the Device for Long‐Term Use
8:00
Results: Correlation of Measured Phenotypes Across Populations in Individual Animals
8:55
Conclusion
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