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Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions

1.6K Views

07:25 min

December 2nd, 2022

DOI :

10.3791/64682-v

December 2nd, 2022


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Caenorhabditis Elegans

Chapters in this video

0:05

Introduction

0:49

Prepare Palmitic Acid Working Solution

1:27

Applying Palmitic Acid Coating to Microtray

2:53

Loading the Microtray with Low‐Melt Nematode Growth Media (lmNGM) and Seeding the Microtray Wells with Bacterial Food

3:47

Enclosing Each Microtray Inside a Single‐Well Plate

4:25

Adding Worms to the Microtray and Preparing the Culture Environment for Long‐Term Use

5:01

Imaging Individual Worms in Microtray Wells

5:27

Results: Correlation of Phenotypes in Individual Animals Across Populations

6:53

Conclusion

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