Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide
941 Views
•
07:34 min
•
May 12th, 2023
DOI :
May 12th, 2023
•1UCD Conway Institute and UCD Institute of Food and Health, School of Agriculture and Food Science, University College Dublin, 2Division of Endocrinology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 3Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 4Department of Pediatrics, University of Cincinnati College of Medicine
Chapters in this video
0:04
Introduction
0:48
Lipid Extraction from Adipose Tissue
2:31
Preparation of Fatty Acid Methyl Esters (FAMEs) and GCMS Analysis
4:08
Deuterium Acetone Exchange of Plasma Samples to Determine Body Water Enrichment
5:04
Results: Plasma Body Water Enrichment from D2O, and Brown Adipose Tissue Total Fatty Acid Enrichment and Synthesis after 3 Days of D2O Administration
6:34
Conclusion
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