Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination
2.1K Views
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09:10 min
•
June 6th, 2022
DOI :
June 6th, 2022
•1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences, Wuyi University
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Chapters in this video
0:04
Introduction
0:51
Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)
2:54
Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)
4:24
Neural Induction (Days 5 7)
5:25
Embedding in the Basement Membrane Matrix (Days 7 10)
6:52
Organoid Maturation (Days 10 40)
7:21
Results: Analysis of Cerebral Organoids
8:41
Conclusion
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