JoVE Journal

Biochemistry

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Nanyang Technological University

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Chapters in this video

0:09

Introduction

1:05

Concentration Determination of RNA Solution

3:15

Hydrolysis of Oligonucleotides of RNA by Xrn-1

5:31

Desalting of RNA Solution, MALDI-TOF Plate Spotting

7:41

Results: Identification of Enzymatically Degraded RNA Fragments Using MALDI-TOF Mass Spectrometry

8:32

Conclusion

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