Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
80.3K Views
•
07:49 min
•
September 9th, 2013
DOI :
September 9th, 2013
•Chapters in this video
0:05
Title
1:09
Recrystallization of Matrices in order to Improve Their Purity (Optional)
2:06
Cleaning of MALDI Stainless Steel Target
2:49
α-CHCA Thin Layer Solution: Preparation and Deposition on the MALDI Target
3:18
Preparation of Matrix Solutions: α-CHCA, DHB, and CHCA_DHB Mixture
4:04
Sample Deposition on the MALDI Target and MALDI Spectra Acquisition
5:17
Results: MALDI-TOF Analysis of Proteins Larger than 100 kDa
7:08
Conclusion
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