JoVE Journal

Biochemistry

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Nanyang Technological University

Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry

High-resolution respirometry coupled to fluorescence sensors determines mitochondrial oxygen consumption and reactive oxygen species (ROS) generation. The present protocol describes a technique to assess mitochondrial respiratory rates and ROS production in the permeabilized sciatic nerve.

Chapters in this video

0:04

Introduction

1:19

Calibration of Polarographic Oxygen Sensors for High-Resolution Respirometry (HRR)

1:56

Dissection and Permeabilization of the Sciatic Nerve

2:58

Oxygen Consumption and Reactive Oxygen Species (ROS) Production Determination

5:51

Results: Assessing the Mitochondrial Oxygen Consumption by the Permeabilized Sciatic Nerve

7:41

Conclusion

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