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Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

2.7K Views

10:42 min

June 17th, 2022

DOI :

10.3791/63223-v

June 17th, 2022


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Toehold Switch Diagnostics

Chapters in this video

0:04

Introduction

1:07

Computational Design of Toehold Switches

2:45

Construction of Toehold Switches by PCR

3:32

Initial Screening of the Switches

5:16

NASBA Primer Screening and Sensitivity

6:55

RT-qPCR for Zika Virus Detection

8:08

Results: Evaluation of Toehold Switches for Paper-Based Zika Sensors

10:01

Conclusion

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