Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
6.4K Views
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09:53 min
•
September 13th, 2021
DOI :
September 13th, 2021
•1Department of Biochemistry, University of Wisconsin, Madison, 2Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, 3Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, 4Department of Chemistry, University of Wisconsin, Madison, 5Morgridge Institute for Research
Chapters in this video
0:04
Introduction
1:25
Preparation of Grids for Patterning and Application of the Anti-fouling Layer
2:50
Applying PLPP Gel
3:33
Micropatterning
6:11
Deposition of Extracellular Matrix (ECM) Proteins
6:54
Results: Micropatterning to Direct Cell Positioning within Whole-cell Cryo-electron Tomography
9:17
Conclusion
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