JoVE Journal
Medicine
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Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease
2.5K Views
•
12:15 min
•
February 8th, 2022
DOI :
February 8th, 2022
•Néstor Pallares-Lupon1,2, Jason D. Bayer1,2, Bastien Guillot1,2, Guido Caluori1,2, Girish S. Ramlugun1,2, Kanchan Kulkarni1,2, Virginie Loyer1,2, Stephane Bloquet1,2, Dounia El Hamrani1,2, Jérôme Naulin1,2, Marion Constantin1,2, Pierre Dos Santos1,2,3, Olivier Bernus1,2, Pierre Jaïs1,2,3, Philippe Pasdois1,2, Richard D. Walton1,2
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Chapters in this video
0:04
Introduction
0:54
Solution Preparation
2:05
Tissue Preparation
5:21
Tissue Dehydration and Drying
6:57
MicroCT
9:20
Results: Contrast-Enhanced Air-Dried Tissue Preparations Enabled Microscale Imaging of Intact Large Mammalian Cardiac Models with Chronic Disease
11:36
Conclusion
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