Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

3.8K Views

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09:31 min

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September 17th, 2021

DOI :

10.3791/62880-v

September 17th, 2021

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Aline Stedman¹^,³, Antonin Levy¹^,⁴, Philippe J. Sansonetti¹^,²^,⁵, Giulia Nigro¹^,⁶

¹Molecular Microbial Pathogenesis Unit, Institut Pasteur, ²Chaire de Microbiologie et Maladies Infectieuses, Collège de France, ³Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit, Sorbonne Université, CNRS UMR7622, INSERM U1156, ⁴Molecular Radiotherapy, INSERM U1030, Gustave Roussy, Université Paris-Saclay, ⁵The Center for Microbes, Development and Health, Institut Pasteur Shanghai and Chinese Academy of Sciences, ⁶Microenvironment and Immunity Unit, Institut Pasteur

Summary

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Oxidative Stress

Murine Intestinal Organoids

Reactive Oxygen Species

Intestinal Content Cleaning

Chapters in this video

0:05

Introduction

1:07

Intestinal Organoids Culture

4:13

Visualization of Oxidative Stress in 3D Organoids by Confocal Microscopy

5:42

Quantification of the Oxidative Stress on the Dissociated Organoids Using Flow Cytometer

7:19

Results: Detection of ROS in the Intestinal Murine Organoids Using Qualitative Imaging and Quantitative Cytometry Assays

8:36

Conclusion

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