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Nanyang Technological University

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Chapters in this video

0:04

Introduction

0:56

Perform Layer‐by‐Layer Deposition of a Prime Layer, DNA Plasmids, and Silk Layers

4:44

Dissolution of SiO2Cores and Imaging of Silk Fibroin Microcapsules

5:42

Estimation of the Permeability of Hollow Microcapsules Using Molecular Weight Cut‐Off (MWCO) Method

6:42

In Vitro Activation of Synthetic Theophylline Riboswitch in Silk Microcapsules

7:28

Results: Microscopy Studies, Membrane Permeability, and Gene Expression Kinetics of Silk Fibroin Microcapsules

9:25

Conclusion

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