This work was supported by LRIR 16RH3003J grant from the Air Force Office of Scientific Research, as well as the Synthetic Biology for Military Environments Applied Research for the Advancement of S&#38;T Priorities (ARAP) program of the U.S. Office of the Under Secretary of Defense for Research and Engineering.
The plasmid vector sequence for ThyRS (pSALv-RS-GFPa1, 3.4 kb) was generously provided by Dr. J. Gallivan. Silkworm cocoons from Bombyx mori were generously donated by Dr. D.L. Kaplan from Tufts University, MA.

1. Construction of plasmid vector.
<ol>
	<li>Construct a plasmid vector (pSALv-RS-GFPa1, 3.4 kb) by amplification of the coding sequence of a theophylline riboswitch (ThyRS) coupled with GFPa1 from pJ201:23976-RS-GFPa1 vector (designed and created by DNA2.0) and insertion into E. coli expression vector, pSAL13. Use forward (5&#39;-CGTGGTACCGGTGATACCAGCATCGTCTTGATG-3&#39;) and reverse (5&#39;-CGTGCTCAGCTTAAGCCAGCTCGTAG-3&#39;) primers to amplify the coding sequence of ThyRS coupled with GFPa1 and perform a PCR reaction in 50 &#181;L volume using DNA polymerase according to the manufacturer&#39;s protocol14.</li>
	<li>Prepare a 1% agarose gel from 0.5 g of agarose, 50 mL of TAE buffer (40 mM Tris Acetate, 1 mM EDTA, pH 8.0), and 3 &#181;L of DNA stain.</li>
	<li>Mix 5 &#181;L aliquot of the PCR-amplified product with 5 &#181;L of RNase/DNase-free water and 2 &#181;L of 6x gel loading dye and analyze by agarose gel electrophoresis. Load a DNA ladder (0.1-10.0 kb) as a reference. Run the gel at 120 V until the dye line has almost reached the bottom of the gel.</li>
	<li>Visualize the DNA fragments using a UV transilluminator imaging system to check for the correct size of DNA15.</li>
	<li>Purify the PCR product using a PCR purification kit according to the manufacturer&#39;s protocol16.</li>
	<li>Digest the PCR product and pSAL expression vector with KpnI and BlpI restriction enzymes in a 15 &#181;L reaction, containing 10 &#181;L of PCR product or plasmid vector (concentration 20-50 ng/&#181;L), 1.5 &#181;L of 10x enzyme buffer, 1 &#181;L of each enzyme, and 1.5 &#181;L of RNase/DNase-free water, at 37 &#176;C for 2 h.</li>
	<li>Add 3 &#181;L of 6x gel loading dye to the reaction mixture and separate the digested fragments on a 1% agarose gel as described in steps 1.3-1.5.</li>
	<li>Purify the DNA fragments using a gel extraction kit according to the manufacturer&#39;s protocol16.</li>
	<li>Ligate the digested PCR product into a digested linearized plasmid vector, pSAL, using T4 DNA ligase and supplemented ligase buffer in a 10 &#181;L reaction containing 3-20 fmol of the digested vector, 9-60 fmol of the digested PCR product, 2 &#181;L of ligase buffer, 1 &#181;L (1 unit) of T4 DNA Ligase, and DNase/RNase-free water. Incubate the ligation reaction at 25 &#176;C for 3 h. 
	NOTE: Ensure that the total DNA content in the reaction mixture is 0.01-0.1 &#181;g.</li>
	<li>Transform E. coli DH5&#945; competent cells with 10 ng of the ligation reaction mixture according to the manufacturer&#39;s protocol17.</li>
	<li>Grow the transformed cells at 37 &#176;C overnight on LB-agar plates supplemented with ampicillin (100 &#181;g/mL).</li>
	<li>Pick 3-4 bacterial colonies from the plate and aseptically transfer each of them into 5 mL of LB media supplemented with ampicillin (100 &#181;g/mL). Grow the cultures overnight at 37 &#176;C in a shaking incubator at 225 rpm.</li>
	<li>Pellet the overnight cultures by centrifugation at 11 x g for 3 min at room temperature.</li>
	<li>Use a purification kit to purify the plasmids according to the manufacturer&#39;s protocol16.</li>
	<li>Verify the sequences of the purified plasmids by DNA sequencing. The plasmid map and the sequence of the resulting construct (pSALv-RS-GFPa1) are shown in Figure 1.</li>
</ol>
2. Large-scale DNA purification.
<ol>
	<li>Transform the plasmid vector pSALv-RS-GFPa1 (3.4 kb) (encoding theophylline riboswitch coupled with GFPa1 reporter gene) or pET28c-F30-2xBroccoli (5.4 kb) (encoding Broccoli aptamer) into E. coli DH5&#945; competent cells according to the manufacturer&#39;s protocol17.</li>
	<li>Grow the transformed cells at 37 &#176;C overnight on LB-agar plates supplemented with ampicillin (100 &#181;g/mL) for cells transformed with pSALv-RS-GFPa1 or kanamycin (50 &#181;g/mL) for cells transformed with pET28c-F30-2x Broccoli.</li>
	<li>Pick 3-4 bacterial colonies from the plate and aseptically transfer each colony into 5 mL of LB media supplemented with appropriate antibiotic (100 &#181;g/mL ampicillin or 50 &#181;g/mL kanamycin). Grow the cultures overnight at 37 &#176;C in a shaking incubator at 225 rpm.</li>
	<li>Use 3 mL of the overnight culture to inoculate into 150 mL of LB supplemented with appropriate antibiotic (100 &#181;g/mL ampicillin or 50 &#181;g/mL kanamycin) and grow the cultures overnight at 37 &#176;C in a shaking incubator at 225 rpm.</li>
	<li>Pellet the cells by centrifugation at &#8805;3400 x g for 10 min at 4 &#176;C.</li>
	<li>Use a purification kit to purify the plasmids according to the manufacturer&#39;s protocol16.</li>
	<li>Elute the DNA with 0.5 mL of pure DNase/RNase-free water. Measure the DNA concentration and prepare 1 mL of DNA stock solutions (100 ng/&#181;L). Store the tubes with DNA at 4 &#176;C until further use.</li>
</ol>
3. Extraction of silk fibroin and preparation of initial materials.
<ol>
	<li>Prepare an aqueous solution of reconstituted silk fibroin (SF) protein from Bombyx mori silkworm cocoons according to the procedure described in detail elsewhere to account for 10% of Silk-LiBr solution18.</li>
	<li>Determine the final concentration of the aqueous SF solution. Pipette 0.5 mL of silk solution to a 60 mm Petri dish, let it dry at 60 &#176;C, and measure the weight of dry silk film. Divide the dry weight by 0.5 mL to calculate the weight per volume percentage.</li>
	<li>Dilute the concentrated silk solution with DNase/RNase-free distilled water by slowly adding water via serological pipette to obtain 1 mg/mL final concentration. Store the solution at 4 &#176;C for future use.</li>
	<li>Prepare fluorescently labeled silk fibroin using an antibody labeling kit. Use 1 mL of 2 mg/mL silk fibroin solution to couple the N-terminal &#945;-amino groups of the protein with an NHS ester-activated derivative dye according to the manufacturer&#39;s protocol19.</li>
	<li>Prepare 50 mL of polyethyleneimine (PEI) aqueous solution with a concentration of 6 mg/mL, adjust pH to 4 with HCl (1 M). Filter the solution through a sterile 0.2 &#181;m membrane. Storage is possible at ambient conditions for months.</li>
	<li>Prepare SiO2 cores. Pipette 300 &#181;L of SiO2 particles into a 2 mL microcentrifuge tube. Wash the microparticles two times with 1 mL of DNase/RNase-free water by centrifugation at 0.2 x g for 1 min.</li>
</ol>
4. Perform Layer-by-Layer deposition of a prime layer, DNA plasmids, and silk layers.
<ol>
	<li>To deposit the PEI prime layer onto the SiO2 microparticles, add 1 mL of PEI solution to the spun down pellet from step 3.6 and agitate the mixture at ambient conditions on a thermomixer at 800 rpm for 15 min. Wash the particles four times with 1 mL of DNase/RNase-free deionized water by centrifugation at 0.2 x g for 1 min.</li>
	<li>To perform deposition of the DNA layer, add 1 mL of the aqueous solution of DNA plasmids from step 2.7 to the PEI-primed microparticles and gently agitate the mixture at 4 &#176;C on a thermomixer at 800 rpm for 15 min. To prepare microcapsules with different DNA loads, adjust the concentration of DNA plasmids from 50-200 ng/&#181;L using DNase/RNase-free distilled water and use 1 mL of these solutions to deposit the DNA. Collect the microparticles by centrifugation at 0.2 x g for 1 min.</li>
	<li>Mark the tubes for DNA plasmids encoding theophylline riboswitch coupled with GFPa1 as ThyRS-GFPa1, and DNA plasmids encoding Broccoli aptamer as BrocApt. 
	NOTE: Keep the microcentrifuge tubes with DNA on ice.</li>
	<li>Carefully remove the supernatant and wash the microparticles four times with 1 mL of DNase/RNase-free distilled water, each time discarding the supernatant after centrifugation at 0.2 x g for 1 min. Perform all experiments at room temperature (RT) unless it is specified otherwise.</li>
	<li>To perform deposition of the silk fibroin layer add 1 mL of the reconstituted aqueous SF solution from step 3.3 to the DNA-adsorbed microparticles, gently vortex and agitate the mixture at 10 &#176;C on the thermomixer at 750 rpm for 15 min. Collect the microparticles by centrifugation at 0.2 x g for 1 min at 4 &#176;C, remove the supernatant, and then wash them once with 1 mL of DNase/RNase-free distilled water. Repeat the centrifugation and discard the supernatant. 
	NOTE: During the experiment, keep the solution of silk on ice to avoid temperature-induced gelation.</li>
	<li>Gradually treat the particles with methanol to induce &#946;-sheet formation in silk protein structure. First, add 0.5 mL of DNase/RNase distilled water, vortex the microcentrifuge tube, then add 0.5 mL of 100% methanol. Gently shake the particles on the thermomixer at 10 &#176;C for 5 min. Collect the particles by centrifugation at 0.2 x g for 1 min. Remove the supernatant.</li>
	<li>Treat the particles with methanol to promote the formation of &#946;-sheets and ensure strong physical adsorption of the silk layer. Add 1 mL of 100% methanol. Gently shake the particles on the thermomixer at 750 rpm for 10 min at 10 &#176;C.</li>
	<li>Collect the particles by centrifugation at 0.2 x g for 1 min at 4 &#176;C and wash them twice with 1 mL of DNase/RNase-free distilled water each time, discarding the supernatant and gently vortexing before the next centrifugation.</li>
	<li>Repeat steps 4.5-4.8 20 times to obtain silk multilayered core-shell structures. For the last deposition step, use fluorescently labeled silk from step 3.4 (Silk-DyLight550, 1 mL).</li>
	<li>Perform the last washing step and keep the microparticles in 1 mL of DNase/RNase-free distilled water at ambient conditions. 
	NOTE: To avoid aggregation of particles during deposition of silk layers, perform a visual inspection of the particles suspension and pipette it up and down using a 1 mL pipette tip to promote homogeneous particle distribution.</li>
	<li>Calculate the number of DNA plasmid copies encapsulated in each microcapsule, NDNA using Equation 1: 
	<img alt="Equation 1" src="/files/ftp_upload/62854/62854eq01.jpg" /> (1) 
	Where N = 6.769 &#215; 1011 - the number of SiO2 cores used for encapsulation. Calculate it from a standard curve for known concentrations of silica particles using serial dilutions and absorption A320 at &#955; = 320 nm; 
	C- initial concentration of DNA used for adsorption 
	V- the volume of DNA used for adsorption 
	0.8- DNA adsorption efficiency on the cores 
	Mw- Molecular weight of DNA plasmid 
	NA- Avogadro&#39;s number (6.022&#160;&#215; 1023)</li>
</ol>
5. Dissolution of cores to obtain silk microcapsules.
<ol>
	<li>Prepare 8% hydrofluoric acid (HF) solution, pH 5.5, by diluting stock solution (48%) with distilled water. Acquire a 50 mL centrifuge tube. Carefully pipette 5 mL of HF and add 25 mL of distilled water to obtain 8% HF solution. 
	CAUTION: HF is a highly corrosive acid and may cause severe burns to the tissues. Extreme caution must be taken during the handling and use of HF for the experiments. Adhere to Standard Operating Procedure (SOP) for the proper usage and handling of HF developed by the organization to avoid undesirable spillage accidents. Do not use glass containers to dilute HF acid. Use the chemical hood to perform this step of the protocol.</li>
	<li>Dissolve SiO2 cores by adding 1.5 mL of 8% HF solution to pelleted core-shell microparticles from step 4.10. Gently vortex and let cores dissolve overnight at ambient conditions with gentle shaking at 450 rpm. 
	NOTE: To avoid spillage of HF, use grafting tape to seal the microcentrifuge tube. Use a chemical hood to perform this step of the protocol.</li>
	<li>Prepare a 2 L glass beaker filled with 2 L of deionized water. Transfer microcapsules solution to dialysis devices (50 kDa MWCO) and dialyze them against deionized water with a repeated change of the water every 3 h for the next 3 days. 
	NOTE: Collect the supernatant during the first three exchanges of water and discard the solution according to the established protocol for hazardous waste materials.</li>
	<li>Use a 1 mL pipette to transfer the suspension from dialysis devices into new 2 mL microcentrifuge tubes to collect the microcapsules. 
	NOTE: Store the aqueous solutions of microcapsules at ambient conditions for several years.</li>
</ol>
6. Imaging of silk fibroin microcapsules using confocal laser scanning microscope (CLSM).
<ol>
	<li>Perform DNA staining using a DNA dye.
	<ol>
		<li>Transfer 300 &#181;L of hollow silk fibroin microcapsules into a fresh 1 mL microcentrifuge tube. Add 500 &#181;L of RNase/DNase-free distilled water.</li>
		<li>Add 5 &#181;L of the DNA staining dye, briefly vortex, and incubate at RT for 2 h protected from light.</li>
		<li>Perform four washing steps by centrifugation at 0.1 x g for 20 min at 4 &#176;C each time, carefully removing 400 &#181;L of the supernatant and replenishing it with 400 &#181;L of RNase/DNase-free distilled water.</li>
	</ol>
	</li>
	<li>Perform the imaging of silk capsules on inverted confocal systems equipped with three major lasers (405 nm, 488 nm, 561 nm) using 100x oil-immersion objective (NA 1.49). Transfer 100 &#181;L of the capsule sample into a single well of 8-well chambered glass slides, allow for capsules to sediment for 20-30 min prior to imaging. 
	NOTE: Dyes are very sensitive to photobleaching. Protect the samples by covering the slides with aluminum foil.</li>
</ol>
7. Estimation of the permeability of hollow microcapsules using molecular weight cut-off (MWCO) method.
<ol>
	<li>Prepare 2 mL each of FITC-labeled dextran fluorophore solutions (20 &#181;M, diH2O) of different Mw (4 kDa, 20 kDa, 40 kDa, 70 kDa, 150 kDa, 250 kDa, 500 kDa, and 2 MDa).</li>
	<li>Pipette 100 &#181;L of the suspension of capsules into a single well of a chambered glass slide. Analyze each microcapsule design (concentration of PEI, loading number of DNA plasmids, the concentration of silk fibroin, and the number of layers) separately.</li>
	<li>To each well, add 300 &#181;L of the specific fluorophore solution starting from the lowest Mw&#160;up to the highest, so each well will correspond to the specific fluorophore solution. Mix by pipetting up and down and let the mixture incubate for 1 h at RT until the diffusion of fluorophore solutions reaches equilibrium.</li>
	<li>Transfer the slide to a confocal laser scanning microscope (CLSM) and image each well using 100x oil-immersion objective at excitation &#955; = 488 nm.
	<ol>
		<li>Identify the area of interest by adjusting the focal plane to make sure that the capsules appear in the form of circles of the largest diameter. This typically happens when viewing the samples closer to the bottom of the well when capsules sediment due to gravity.</li>
		<li>Collect several images of microcapsule samples by moving the slide in XY direction. Capture images to account for up to 100-150 capsules for each sample.</li>
		<li>Use ImageJ software to analyze the permeability of the capsule&#39;s membrane in each Mw fluorophore solution by comparing fluorescence intensities inside and outside of the capsules. For that, draw a region of interest (ROI) in the form of a circle to outline the circumference of the capsule and click Analyze/ Measure to measure the fluorescence intensity inside. Tabulate the data into a spreadsheet. Perform this operation for each microcapsule for a total of 200-300 capsules.</li>
		<li>Assess the outside fluorescence intensity in the same way by outlining the ROI and measuring the intensity away from the capsules. Perform 3-5 measurements for statistical analysis.</li>
		<li>To perform statistical analysis, compare the fluorescence intensities inside and outside of the capsules using paired t-test (p &#60; 0.05).</li>
		<li>Use the conversion Table 2 to estimate the permeability of microcapsules based on the hydrodynamic radii for FITC-Dextran with variable Mw.</li>
	</ol>
	</li>
</ol>
8. In vitro activation of synthetic theophylline riboswitch in silk microcapsules
<ol>
	<li>Prepare 1 mL of theophylline stock solution (100 mM, DMSO). Prepare E. coli S30 extract system for circular DNA by thawing the components on ice for 40 min.</li>
	<li>Obtain a 0.5 mL DNase/RNase-free microcentrifuge tube. Perform in vitro transcription/translation reaction, combine the cell-free components with a sample of microcapsules in the following order (50 &#181;L total volume): S30 premix without amino acids (20 &#181;L); S30 extract, circular (15 &#181;L); complete amino acids mixture (5 &#181;L); hollow microcapsules containing ThyRS-GFPa1 plasmids from step 4.10 (9 &#181;L); and theophylline, 100 mM DMSO (1 &#181;L). 
	NOTE: After adding all components, briefly vortex the tube and collect the sample during brief centrifugation at 0.2 &#215; g for a couple of seconds.</li>
	<li>Incubate the tube at 30 &#176;C for 4 h and check the fluorescence on a plate reader using excitation at &#955; = 488 nm and emission for GFP/FITC filter (510 nm &#177; 20 nm).</li>
	<li>Image the capsules on any LCSM system using 488 nm and 561 nm lasers. Obtain the best quality images using 100x oil immersion objective and 8-well chambered slides.</li>
</ol>
9. In vitro activation of broccoli aptamer in silk microcapsules
<ol>
	<li>Prepare 1 mL of stock solution of DFHBI-1T dye (30 &#181;M, diH2O). Prepare the PURE (protein synthesis using recombinant elements) cell-free system reaction kit by thawing the components on ice for 40 min.</li>
	<li>Obtain a 0.5 mL DNase/RNase-free microcentrifuge tube. Perform in vitro transcription reaction by combining cell-free reaction components with a sample of microcapsules in the following order (50 &#181;L total volume): solution A (20 &#181;L); solution B (15 &#181;L); hollow microcapsules containing BrocApt plasmids from step 4.10 (14 &#181;L); and DFHBI-1T dye (1 &#181;L). 
	NOTE: After adding all components, briefly vortex the tube and collect the sample during brief centrifugation at 0.2 &#215; g for a couple of seconds.</li>
	<li>Incubate the tube at 37 &#176;C for 6 h and check the fluorescence on a plate reader using excitation at &#955;ex = 470 nm and emission at &#955;em = 510 nm &#177; 20 nm.</li>
	<li>Image the capsules on any LCSM system using 488 nm and 561 nm lasers. Obtain the best quality images using 100x oil-immersion objective, and 8-well coverglass chambered slides.</li>
</ol>

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L., Kelley-Loughnane, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improving+the+activity+of+DNA-encoded+sensing+elements+through+confinement+in+silk+microcapsules.">Improving the activity of DNA-encoded sensing elements through confinement in silk microcapsules.</a> ACS Applied Materials &amp; Interfaces. 12 (43), 48329-48339 (2020).</li><li>Melnikov, S., Ben-Shem, A., Garreau de Loubresse, N., Jenner, L., Yusupova, G., Yusupov, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Structural+basis+for+the+inhibition+of+the+eukaryotic+ribosome.">Structural basis for the inhibition of the eukaryotic ribosome.</a> Nature Structural &amp; Molecular Biology. 19 (6), 560-567 (2012).</li><li>Zhao, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+future+of+layer-by-layer+assembly:+A+tribute+to+ACS+Nano+associate+editor+Helmuth+M&#246;hwald.">The future of layer-by-layer assembly: A tribute to ACS Nano associate editor Helmuth M&#246;hwald.</a> ACS Nano. 13 (6), 6151-6169 (2019).</li><li>Main, K. H. S., Provan, J. I., Haynes, P. J., Wells, G., Hartley, J. A., Pyne, A. L. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Atomic+force+microscopy-A+tool+for+structural+and+translational+DNA+research.">Atomic force microscopy-A tool for structural and translational DNA research.</a> APL Bioengineering. 5, 031504 (2021).</li><li>Riera, R., Feiner-Gracia, N., Fornaguera, C., Cascante, A., Borr&#243;s, S., Albertazzi, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tracking+the+DNA+complexation+state+of+pBAE+polyplexes+in+cells+with+super+resolution+microscopy.">Tracking the DNA complexation state of pBAE polyplexes in cells with super resolution microscopy.</a> Nanoscale. 11 (38), 17869-17877 (2019).</li><li>Bilokapic, S., Strauss, M., Halic, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cryo-EM+of+nucleosome+core+particle+interactions+in+trans.">Cryo-EM of nucleosome core particle interactions in trans.</a> Scientific Reports. 8, 7046 (2018).</li><li>Pritchard, E. M., Dennis, P. B., Omenetto, F., Naik, R. R., Kaplan, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Physical+and+chemical+aspects+of+stabilization+of+compounds+in+silk.">Physical and chemical aspects of stabilization of compounds in silk.</a> Biopolymers. 97 (6), 479-498 (2012).</li><li>Fritz, B. R., Jamil, O. K., Jewett, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Implications+of+macromolecular+crowding+and+reducing+conditions+for+in+vitro+ribosome+construction.">Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction.</a> Nucleic Acids Research. 43 (9), 4774-4784 (2015).</li><li>Ge, X., Luo, D., Xu, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell-free+protein+expression+under+macromolecular+crowding+conditions.">Cell-free protein expression under macromolecular crowding conditions.</a> PLoS One. 6 (12), 28707 (2011).</li><li>Cawte, A. D., Unrau, P. J., Rueda, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Live+cell+imaging+of+single+RNA+molecules+with+fluorogenic+mango+II+arrays.">Live cell imaging of single RNA molecules with fluorogenic mango II arrays.</a> Nature Communications. 11, 1283 (2020).</li><li>Chen, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visualizing+RNA+dynamics+in+live+cells+with+bright+and+stable+fluorescent+RNAs.">Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.</a> Nature Biotechnology. 37 (11), 1287-1293 (2019).</li></ol>

The views and opinions presented herein are those of the authors and do not necessarily represent the views of DoD or its Components

Selectively permeable hydrogel microcapsules loaded with various types of DNA-encoded sensor designs can be prepared following this protocol. One of the distinctive features of the LbL approach is the ability to tailor the complexity of microcapsules during the bottom-up assembly, which usually starts with the adsorption of molecular species on sacrificial templates. By carefully adjusting concentrations of the initial components, pH conditions, and the number of layers, microcapsules with different DNA loading parameters, functionality, and tunable permeability can be prepared23. In order to amplify the versatility of the capsules, further functionalization of the shell surface with AuNPs and IgG can be achieved to implement biocompatible sensor carriers for in vivo diagnostics. Both of these alternative routes rely on the unique molecular structure of the silk fibroin. In situ reduction of Au3+ ions can be accomplished due to the presence of tyrosine residues (5.3% mol.) capable of reducing metal ions in the presence of an optimal reduction buffer. The conjugation of specific antibodies to the surface of AuNPs-functionalized protein microcapsules can be done via the implementation of carbodiimide activation chemistry. Both of these steps require careful development and optimization of the protocol in order to achieve the proper functionality of the biosensor capsules for future applications. For instance, to use these microcapsules as &#34;smart&#34; delivery systems, in which a molecular cargo is delivered upon specific stimuli (like pH or chemical cues), specific properties of these capsules should be characterized and tuned, including delivery efficiency, retention time, route of administration and disease model treatments.
The DNA-loaded microcapsules were characterized by the CLSM technique and fluorescence measurements. However, other characterization methods such as atomic force microscopy (AFM), cryogenic electron tomography (CEM), and direct stochastic reconstruction super-resolution microscopy (dSTORM) can be applied to differentiate specific structures of the microcapsules, including individual fibers, nanodomains, and localization of DNA plasmids24,25,26.
The developed protocol highlights the use of silk fibroin biopolymer as a biocompatible network material that preserves the tertiary structure of loaded DNA plasmids. The stability of immobilized DNA sequences within the silk fibroin network occurs via hydrophobic interactions and/or hydrogen bonding, which provide a protective microenvironment against pH inactivation, oxidation or hydrolysis27. In addition, the &#946;-crystalline domains of silk fibroin provide a unique mechanical barrier, restricting the movement of entrapped macromolecules, and preventing DNA plasmids from degradation during storage in aqueous solutions.
The semi-permeable nature of silk fibroin microcapsules loaded with DNA templates created unique spatial and physicochemical conditions for the IVTT reactions. Transcriptionally and translationally activated RNA aptamer and riboswitch immobilized in silk fibroin microcapsules were able to produce an output signal that was at least three-fold higher in comparison to free non-immobilized sensors. In addition, the immobilization of DNA templates in microcapsules can significantly enhance the activity of encapsulated sensors beyond the detection limit of non-encapsulated ones. While non-encapsulated sensors had a minimal response at low DNA concentrations, the encapsulated sensors had a very distinct output response, which can be easily titrated to reflect the subtle changes in DNA concentrations. The improved response signal of encapsulated reporters was likely due to unrestricted diffusion of the components of IVTT machinery and reduced mobility of DNA plasmids. Several studies have recognized that the yield of protein synthesis in cell-free reactions is dependent upon the macromolecular environment28,29. Immobilization of DNA plasmids into silk network provided an effective confined environment restricting oligonucleotides movement and accelerating transcription/translation mechanism reactions even from several DNA copies21.
While the LbL method provides a very versatile approach to construct multifunctional assemblies in a controllable manner, the fabrication procedure is relatively time-consuming and typically requires processing adjustments to scale up the microcapsules yield. Another consideration in making biomolecule-based microcapsules is the compatibility of any given system with the requirement for the core dissolution after LbL deposition is completed. So far, to produce homogeneous in size and robust hollow silk-based DNA-laden microcapsules, the shells were deposited on sacrificial silica (SiO2) core templates, which required subsequent removal by hydrofluoric (HF) acid etching. Nor HF handling or etching are considered biocompatible processes, limiting their widespread use in practical applications. The alternative to SiO2 inorganic colloidal templates, carbonate cores are typically inert in forming complexes with polymer layers and can be dissolved under mild conditions using ethylenediaminetetraacetic acid (EDTA). However, adjusting sacrificial cores may affect the deposition properties of multilayers and the integrity of the shell structure, which requires further optimization of the protocol.
In summary, the current protocol allows the preparation of silk-based DNA-laden microcapsules with different sensor designs. The combination of a confined microenvironment provided by the LbL method and the use of silk fibroin as a biocompatible material improved the sensing properties of encapsulated DNA. With the rapid development of fluorogenic RNA aptamer technology, the potential use of DNA-laden silk microcapsules can be extended to multiplexed in vitro diagnostics. Recently, several variations of RNA-based fluorescent aptamers have emerged as powerful background-free technology for imaging RNA in live cells with high signal-to-noise ratio sensitivity30,31. By applying synthetic RNA nanotechnology to design artificial RNA aptamers, the fluorogenic properties of aptamers can be harnessed to detect specific ligands of interest. This technology will be specifically valuable to monitor biomarkers of interest in different formats, including point-of-use paper-based sensors, hydrogel-based patches for sweat or interstitial fluid, and implantable materials.

The field of synthetic biology offers unique opportunities to develop sensing capabilities by exploiting natural mechanisms evolved by microorganisms to monitor their environment and potential threats. Importantly, these sensing mechanisms are typically linked to a response that protects these microorganisms from harmful exposure, regulating gene expression to mitigate negative effects or prevent intake of toxic materials. There have been significant efforts to engineer these microorganisms to create whole-cell sensors taking advantage of these natural responses but re-directing them to recognize novel targets and/or to produce a measurable signal that can be measured for quantification purposes (typically fluorescence)1,2. Currently, concerns with the use of genetically modified microorganisms (GMOs), especially when releasing in the environment or the human body, due to leakage of whole cells or some of their genetic material, even if encapsulated in a polymer matrix, suggest that alternative ways to exploit these sensing approaches are needed3.
A powerful approach to exploit the benefits of microorganisms-based sensing without the concern for the deployment of GMOs is the use of in vitro transcription/translation (IVTT) systems. From a practical perspective, IVTT systems consist of a mixture containing most of the cell components in an active state that has been &#34;extracted&#34; from cells by different means, including sonication, bead-beating, or others4. The final product of this process is a biochemical reaction mixture already optimized to perform transcription and translation that can be used to test different sensors in an &#34;open vessel&#34; format, without the constraints associated with the use of whole cells (membrane diffusion, transformation efficiency, cell toxicity, etc.). Importantly, different sensor components can be quantitatively added, and their effect studied by different optical and spectrometric techniques, as we have demonstrated5. It has been noticed that the performance of IVTT systems can be inconsistent; however, recent studies have shown approaches to standardize their preparation and characterization, which is of great help when studying their performance in sensor design6. Recently, many examples of IVTT systems using to create paper-based assays through the lyophilization of their components in paper matrices have been demonstrated, including detection of heavy metal ions, drugs, quorum sensing elements, and others7,8,9. An exciting application space for IVTT-based sensors is their use in sensing applications in different types of environments, including soil, water, and the human body. In order to deploy these IVTT systems to these challenging environments, an encapsulation approach need to be implemented to contain the IVTT components and protect them from degradation.
The most common encapsulation approaches for IVTT systems include the use of lipid capsules, micelles, polymersomes, and other tightly enclosed microcontainers10,11,12. One disadvantage of this approach is the need to incorporate either passive or active mechanisms to transport materials in and out of the containers to allow communication with the external environment and provide sensing capabilities. To overcome some of these issues, the study here reports a method that provides a simple yet effective approach to encapsulate the encoding materials for different sensor designs to be expressed in IVTT systems. This approach is based on the use of Layer-by-Layer (LbL) deposition of a biopolymer in the presence of the plasmids of interest to create hollow microcapsules with high porosity, which allows the protected genetic material to interact with the different components of the IVTT of choice. The study demonstrated that encapsulated plasmids could direct transcription and translation when activated within this polymeric matrix, as shown with the response of a plasmid-encoded aptamer and a riboswitch to their corresponding targets. Additionally, this LbL coating protects the plasmids for months without any special storage conditions.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>(Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T)</td>
			<td>Lucerna</td>
			<td>DFHBI-1T</td>
			<td></td>
		</tr>
		<tr>
			<td>5x T4 DNA Ligase Buffer</td>
			<td>ThermoFisher Scientific</td>
			<td>46300-018</td>
			<td></td>
		</tr>
		<tr>
			<td>6x Blue Gel Loading Dye</td>
			<td>New England BioLabs</td>
			<td>B7021S</td>
			<td></td>
		</tr>
		<tr>
			<td>96-well plates, black circular</td>
			<td>Corning</td>
			<td>3601</td>
			<td></td>
		</tr>
		<tr>
			<td>Agarose</td>
			<td>Sigma-Aldrich</td>
			<td>A9539</td>
			<td>BioReagent, for molecular biology, low EEO</td>
		</tr>
		<tr>
			<td>Ampicillin sodium salt</td>
			<td>Sigma-Aldrich</td>
			<td>A0166</td>
			<td>powder or crystals, BioReagent, suitable for cell culture</td>
		</tr>
		<tr>
			<td>BlpI restriction enzymes</td>
			<td>New England BioLabs</td>
			<td>R0585S</td>
			<td></td>
		</tr>
		<tr>
			<td>Corning Disposable Vacuum Filter/Storage Systems</td>
			<td>FisherScientific</td>
			<td>09-761-1</td>
			<td></td>
		</tr>
		<tr>
			<td>Dimethyl sulfoxide, DMSO</td>
			<td>Sigma-Aldrich</td>
			<td>472301</td>
			<td>ACS reagent, &#8805;99.9%</td>
		</tr>
		<tr>
			<td>DNA Plasmid, pET28c-F30-2x Broccoli (5.4 kb), BrocApt.</td>
			<td>Addgene</td>
			<td>Plasmid #66788</td>
			<td></td>
		</tr>
		<tr>
			<td>DyLightTM550 Antibody Labeling kit (Invitrogen)</td>
			<td>ThermoFisher Scientific</td>
			<td>84530</td>
			<td></td>
		</tr>
		<tr>
			<td>E. coli S30 extract system for circular DNA</td>
			<td>Promega</td>
			<td>L1020</td>
			<td></td>
		</tr>
		<tr>
			<td>Falcon Conical centrifuge tubes, 15 mL</td>
			<td>FisherScientific</td>
			<td>14-959-53A</td>
			<td></td>
		</tr>
		<tr>
			<td>Falcon Conical centrifuge tubes, 50 mL</td>
			<td></td>
			<td>14-432-22</td>
			<td></td>
		</tr>
		<tr>
			<td>Fisherbrand Microcentrifuge tubes, 1.5 mL</td>
			<td>FisherScientific</td>
			<td>05-408-129</td>
			<td></td>
		</tr>
		<tr>
			<td>Hydrofluoric acid, HF</td>
			<td>Sigma-Aldrich</td>
			<td>695068</td>
			<td>ACS reagent, 48%</td>
		</tr>
		<tr>
			<td>Kanamycin sulfate</td>
			<td>Sigma-Aldrich</td>
			<td>60615</td>
			<td>mixture of Kanamycin A (main component) and Kanamycin B and C</td>
		</tr>
		<tr>
			<td>KpnI restriction enzymes</td>
			<td>New England BioLabs</td>
			<td>R0142S</td>
			<td></td>
		</tr>
		<tr>
			<td>LB agar plate supplemented with 100 &#181;g/mL ampicillin</td>
			<td>Sigma-Aldrich</td>
			<td>L5667</td>
			<td>pre-poured agar plates with 100 &#181;g/mL ampicillin</td>
		</tr>
		<tr>
			<td>LB agar plate supplemented with 50 &#181;g/mL kanamycin</td>
			<td>Sigma-Aldrich</td>
			<td>L0543</td>
			<td>pre-poured agar plates with 50 &#181;g/mL kanamycin</td>
		</tr>
		<tr>
			<td>LB broth (Lennox grade)</td>
			<td>Sigma-Aldrich</td>
			<td>L3022</td>
			<td></td>
		</tr>
		<tr>
			<td>Lithium bromide, LiBr</td>
			<td>Sigma-Aldrich</td>
			<td>213225</td>
			<td>ReagentPlus, &#8805;99%</td>
		</tr>
		<tr>
			<td>Max Efficiency DH5-&#945; competent E. coli strain</td>
			<td>ThermoFisher Scientific</td>
			<td>18258012</td>
			<td></td>
		</tr>
		<tr>
			<td>Methanol</td>
			<td>MilliporeSigma</td>
			<td>322415</td>
			<td>anhydrous, 99.8%</td>
		</tr>
		<tr>
			<td>MilliQ-water</td>
			<td>EMD MilliPore</td>
			<td></td>
			<td>Milli-Q Reference Water Purification System</td>
		</tr>
		<tr>
			<td>MinElute PCR Purification Kit</td>
			<td>Qiagen</td>
			<td>28004</td>
			<td></td>
		</tr>
		<tr>
			<td>N-(3-Dimethylaminopropyl)-N&#8242;-ethylcarbodiimide hydrochloride, EDC</td>
			<td>Sigma-Aldrich</td>
			<td>E1769</td>
			<td></td>
		</tr>
		<tr>
			<td>PBS (phosphate buffered saline)</td>
			<td>ThermoFisher Scientific</td>
			<td>10010023</td>
			<td>1x PBS, pH 7.4</td>
		</tr>
		<tr>
			<td>Phusion High-Fidelity DNA Polymerase</td>
			<td>New England Biolabs</td>
			<td>M0530S</td>
			<td></td>
		</tr>
		<tr>
			<td>Polyethylenimine, branched</td>
			<td>Sigma-Aldrich</td>
			<td>408727</td>
			<td>average Mw ~25,000</td>
		</tr>
		<tr>
			<td>PURExpress In Vitro Protein Synthesis Kit</td>
			<td>New England BioLabs</td>
			<td>E6800S</td>
			<td></td>
		</tr>
		<tr>
			<td>QIAEX II Gel Extraction Kit</td>
			<td>Qiagen</td>
			<td>20021</td>
			<td></td>
		</tr>
		<tr>
			<td>QIAprep Spin Miniprep Kit</td>
			<td>Qiagen</td>
			<td>&#160;27104</td>
			<td></td>
		</tr>
		<tr>
			<td>Quick-Load 2-Log DNA Ladder (0.1-10.0 kb)</td>
			<td>New England BioLabs</td>
			<td>N0469S</td>
			<td></td>
		</tr>
		<tr>
			<td>SiO&#8322; silica microspheres, 4.0 &#181;m</td>
			<td>Polysciences, Inc.</td>
			<td>24331-15</td>
			<td>10% aqueous solution</td>
		</tr>
		<tr>
			<td>Slide-A-Lyzer G2 Dialysis Cassettes, 3.5K MWCO, 15 mL</td>
			<td>ThermoFisher Scientific</td>
			<td>87724</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium carbonate, Na&#8322;CO&#8323;</td>
			<td>Sigma-Aldrich</td>
			<td>222321</td>
			<td>ACS reagent, anhydrous, &#8805;99.5%, powder</td>
		</tr>
		<tr>
			<td>Spectrum Spectra/Por Float-A-Lyzer G2 Dialysis Devices</td>
			<td>FisherScientific</td>
			<td>08-607-008</td>
			<td>Spectrum G235058</td>
		</tr>
		<tr>
			<td>SYBR Safe DNA gel stain</td>
			<td>ThermoFisher Scientific</td>
			<td>S33102</td>
			<td></td>
		</tr>
		<tr>
			<td>T4 DNA Ligase (5 U/&#181;L)</td>
			<td>ThermoFisher Scientific</td>
			<td>EL0011</td>
			<td></td>
		</tr>
		<tr>
			<td>Theophylline</td>
			<td>Sigma-Aldrich</td>
			<td>T1633</td>
			<td>anhydrous, &#8805;99%, powder</td>
		</tr>
		<tr>
			<td>Tris Acetate-EDTA buffer (TAE buffer)</td>
			<td>Sigma-Aldrich</td>
			<td>T6025</td>
			<td>Contains 40 mM Tris-acetate and 1 mM EDTA, pH 8.3.</td>
		</tr>
		<tr>
			<td>UltraPure DNase/RNase-Free Distilled Water</td>
			<td>FisherScientific</td>
			<td>10-977-023</td>
			<td></td>
		</tr>
		<tr>
			<td>ZymoPURE II Plasmid MaxiPrep kit</td>
			<td>ZymoResearch</td>
			<td>D4202</td>
			<td></td>
		</tr>
	</tbody>
</table>

preparation of multifunctional silk-based microcapsules loaded with dna plasmids encoding rna aptamers and riboswitches

We introduce a protocol for the preparation of DNA-laden silk fibroin microcapsules via the Layer-by-Layer (LbL) assembly method on sacrificial spherical cores. Following adsorption of a prime layer and DNA plasmids, the formation of robust microcapsules was facilitated by inducing &#946;-sheets in silk secondary structure during acute dehydration of a single silk layer. Hence, the layering occurred via multiple hydrogen bonding and hydrophobic interactions. Upon adsorption of multilayered shells, the core-shell structures can be further functionalized with gold nanoparticles (AuNPs) and/or antibodies (IgG) to be used for remote sensing and/or targeted delivery. Adjusting several key parameters during sequential deposition of key macromolecules on silica cores such as the presence of a polymer primer, the concentration of DNA and silk protein, as well as a number of adsorbed layers resulted in biocompatible, DNA-laden microcapsules with variable permeability and DNA loadings. Upon dissolution of silica cores, the protocol demonstrated the formation of hollow and robust microcapsules with DNA plasmids immobilized to the inner surface of the capsule membrane. Creating a selectively permeable biocompatible membrane between the DNA plasmids and the external environment preserved the DNA during long-term storage and played an important role in the improved output response from spatially confined plasmids. The activity of DNA templates and their accessibility were tested during in vitro transcription and translation reactions (cell-free systems). DNA plasmids encoding RNA light-up aptamers and riboswitches were successfully activated with corresponding analytes, as was visualized during localization of fluorescently labeled RNA transcripts or GFPa1 protein in the shell membranes.

Here, the study addresses the functionality of DNA templates encoding different sensor designs (two types of RNA-regulated transcription/translation elements) after encapsulation in silk protein capsules. Microcapsules were prepared via templated Layer-by-Layer (LbL) assembly of the key components: A prime layer, DNA plasmids encoding sensor designs, and silk fibroin biopolymer (Figure 2). Deposition of macromolecules in a layered fashion allows controlling the permeability of the capsule membrane based on inter- and intramolecular interactions between the absorbed layers and the thickness of the shell. The tunable permeability of the system offered the potential to control the diffusion of essential molecules while limiting the diffusion of large undesirable macromolecules through the capsule membrane20.
Homogeneous in size (4.5 &#181;m) and robust silk fibroin microcapsules with a shell thickness of ~500 nm (20 layers) in a hydrated state can be produced when properly following the protocol (Figure 3)21. The LbL approach allowed to tune the loading capacity of DNA templates based on the initial concentration of the plasmids. The optimal DNA loading can be achieved by varying the initial concentration of DNA templates from 50-200 ng/&#181;L. Table 1 represents the number of DNA copies retained in a single microcapsule upon completion of LbL encapsulation and was calculated for each sensor design based on the initial DNA concentrations and Mw of DNA plasmids according to Equation 1. The optimal DNA loading capacity was achieved with 32 and 20 DNA copies for ThyRS and BrocApt sensor designs, respectively. The assessment of loading capacity was important to have an accurate estimation of the encapsulated DNA concentrations to be able to titrate it during IVTT activation of the sensors by adding more concentrated capsule samples.
The permeability of the capsule shells can be systematically analyzed by following the MWCO method. The method estimates the pore size of the membrane based on the molecular weight of solute molecules that could not penetrate through the capsule membrane. By subjecting microcapsules to variable Mw fluorophore molecules and performing confocal imaging in the focal plane that corresponds to the largest diameter across multiple capsules, the permeability of the shell membranes can be assessed. ImageJ analysis allows to estimate the fluorescence intensities inside and outside of the capsules and identify fully permeable (outside and inside fluorophore intensities were comparable), partially permeable (50%-70% of capsules had lower fluorescence inside compared to outside), and not permeable (inside fluorophore intensity was lower compared to outside intensity) membranes (Figure 4). Converting Mw to hydrodynamic radius for each fluorophore allows estimating the mesh size of the capsule membrane (Table 2).
Selective permeability of protein capsules can be attained during systematic optimization of LbL deposition protocol by using variable concentrations of a prime layer (from 0-6 mg/mL), the concentration of a silk fibroin (from 0.5-2 mg/mL) and the number of deposited layers (from 10-25) until optimal permeability is achieved. In this protocol, optimal permeability between 25-32 nm was achieved with 6 mg/mL of PEI as a prime layer and 20 layers of 1 mg/mL of silk fibroin deposited during LbL assembly (Figure 4). This permeability range was ideal for the components of the IVTT system to percolate through the capsule shell. Typically, the largest molecular complexes of any IVTT system are prokaryotic ribosomal units, 30S and 50S, which, when bound by mRNA in a ribosomal complex, can reach up to ~20 nm in size22. The structure of the developed microcapsules shells resembles a highly intertwined network of silk fiber layers that are physically crosslinked by &#946;-sheet blocks produced during LbL assembly. This membrane structure is semi-permeable: highly permeable to small molecules (e.g., ions, amino acids, peptides, sugars, etc.) and restricted to large molecules (e.g., high molecular weight proteins, glycoproteins, cells, etc.) that only allows percolation of molecules with a hydrodynamic radius less than 25 nm. Hence, silk fibroin microcapsules with optimal DNA loading and membrane permeability can be used as bioreactor carriers for IVTT activation.
The transcriptional and translational activity of ThyRS and BrocApt design sensors can be tested using commercially available IVTT systems. ThyRS requires translational activation of the reporter gene in the presence of theophylline ligand. Activation of synthetic ThyRS involves several steps to initiate gene expression, including mRNA synthesis, the conformational change of mRNA sequence upon binding to the analyte, release of the ribosome binding site, and the synthesis of the reporter GFPa1 protein. All these steps require free diffusion of the IVTT components through the capsule membrane. The proposed design of DNA-laden silk fibroin microcapsules improved the activation parameters of RNA-regulated sensor designs: Both the expression kinetics and fluorescence output were significantly higher in comparison to the free non-immobilized DNA (Figure 5A). The CLSM imaging also confirmed successful GFPa1 gene activation within capsules loaded with DNA plasmids encoding ThyRS sequences (Figure 5B-D).
Alternatively, the activation of BrocApt sensor design was carried out in the IVTT system that supports T7 E. coli promoter coupled transcription/translation in the presence of DHFBI-1T dye. The fluorescence output was initiated upon binding the fluorogenic dye to the mRNA sequence. Importantly, the output signal from silk microcapsules was several folds higher compared to non-encapsulated DNA samples of equivalent concentrations (Figure 6). Hence, silk fibroin microcapsules can retain the essential functionality of DNA-encoded sensor elements, which can be important for the design of new types of in vitro biosensor platforms for rapid and sensitive detection of analytes of interest.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/62854/62854fig01.jpg" /> 
Figure 1: Plasmid map and sequence of pSALv-RS-GFPa1. (A) The plasmid contains theophylline riboswitch (ThyRS) sequence placed upstream of GFPa1 encoding gene under the control of ptac promoter, &#946;-lactamase (bla) encoding gene responsible for resistance to ampicillin. (B) The ptac promoter sequence is shown in bold and underlined; theophylline riboswitch sequence is shown in bold italic; GFPa1 encoding sequence is shown in bold; restriction sites sequences are underlined. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/62854/62854fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/62854/62854fig2v2.jpg" /> 
Figure 2: Overview of the preparation of microcapsules using the Layer-by-Layer approach. Pristine SiO2 cores were firstly functionalized with PEI polymer, followed by the sequential deposition of DNA plasmids encoding different sensor designs and silk fibroin layers until the desired number of silk protein layers have been adsorbed. Hollow pristine microcapsules containing various DNA sensor designs can be produced by dissolving sacrificial cores. Activation of each biosensor design encapsulated in the microcapsule can be achieved during IVTT reactions in the presence of corresponding ligands. This figure has been reprinted from Drachuk, I. et al.21. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/62854/62854fig2v2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/62854/62854fig03.jpg" /> 
Figure 3:&#160;Microscopy studies for DNA-loaded microcapsules. (A) Cross-sectional 2D and (B) Rendered 3D confocal microscopy images of hollow (SF)20 microcapsules loaded with DNA plasmids encoding ThyRS (32 DNA copies/capsule) and produced from 6 mg/mL PEI-primed SiO2 cores. Auto-fluorescence from silk fibroin capsules (DAPI channel, blue) and stained DNA (FITC channel, green) were applied to identify the localization of DNA plasmids and estimate the capsule membrane thickness. Inset in (A) represents intensity profiles for DNA and silk fibroin shells across the capsule. Scale bar: 2 &#181;m. Inset in (B) represents the intensity profile for silk protein across the capsule shell. Membrane thickness was measured as the length at half intensity peak. Processing of the cross-sectional images and 3D rendering was performed using NIS imaging software on Nikon C2+ system. This figure has been reprinted from Drachuk, I. et al.21. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/62854/62854fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/62854/62854fig04.jpg" /> 
Figure 4: Estimation of the membrane permeability for silk microcapsules using the MWCO method. Selective CLSM representative fluorescent images of hollow silk fibroin microcapsules subjected to FITC-Dextran solutions of various Mw (20 &#181;M, diH2O). Capsules were prepared with a different number of layers and PEI concentrations. An increase in the concentration of a prime layer leads to increased colloidal stability of microcapsules with more permeable shells, while the elimination of the prime layer causes aggregation of capsules with less permeable shell membranes. Scale bar: 5 &#181;m. This figure has been reprinted from Drachuk, I. et al.21. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/62854/62854fig04large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 5" class="xfigimg" src="/files/ftp_upload/62854/62854fig5v2.jpg" /> 
Figure 5: Activation of ThyRS sensor design in silk fibroin microcapsules. (A) Kinetics for GFPa1 expression during ThyRS activation from DNA plasmids loaded into 20-layered SF microcapsules (red-colored lines) and control non-encapsulated DNA (blue-colored lines). From top to bottom, concentrations of DNA in a sample volume (50 &#181;L) were 6.5 ng/&#181;L, 4.5 ng/&#181;L, 2.5 ng/&#181;L, and 0 ng/&#181;L and correspond to the changes in color intensities. (B) CLSM images of silk fibroin capsules loaded with 32 DNA copies per capsule. Scale bar: 5 &#181;m. (C) The image corresponds to cross-sectional intensity profiles corresponding to image (B) across two capsules (white line in B). The red line represents silk capsules, and the green line represents GFPa1 expressed in capsules. (D) The image represents rendered 3D silk capsules loaded with 32 copies of DNA after incubation in the IVTT system. Green fluorescence corresponds to the GFPa1 signal, and red fluorescence corresponds to fluorescently labeled silk layers. Scale bar: 2 &#181;m. ThyRS activation was performed with theophylline (2 mM, DMSO) during the incubation of microcapsules in the IVTT system (S30 extract). This figure has been reprinted from Drachuk, I. et al.21. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/62854/62854fig5v2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 6" class="xfigimg" src="/files/ftp_upload/62854/62854fig6v2.jpg" /> 
Figure 6: Activation of BroccApt in silk fibroin microcapsules. Comparison of transcription kinetics was performed for 20-layered silk microcapsules loaded with 30 DNA copies per capsule (red-colored lines) and control non-encapsulated DNA (blue-colored lines). From top to bottom, concentrations of encapsulated DNA in a sample were: 3.6 ng/&#181;L, 2 ng/&#181;L, 1 ng/&#181;L and 0 ng/&#181;L and correspond to the changes in color intensities. Concentrations of non-encapsulated DNA were: 20 ng/&#181;L, 10 ng/&#181;L, 5 ng/&#181;L and 0 ng/&#181;L and correspond to the changes in color intensities. Activation was performed with DHBFI-1T (100 &#181;M, diH2O) during incubation in the PURE cell-free system. This figure has been reprinted from Drachuk, I. et al.21. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/62854/62854fig6v2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>DNA&#160; Sensor Design</td>
			<td>Initial DNA Concentration (ng/&#181;L)</td>
			<td>Number of DNA copies per capsule (DNA/capsule)</td>
		</tr>
		<tr>
			<td></td>
			<td>50</td>
			<td>16</td>
		</tr>
		<tr>
			<td>ThyRS</td>
			<td>100</td>
			<td>32</td>
		</tr>
		<tr>
			<td></td>
			<td>150</td>
			<td>48</td>
		</tr>
		<tr>
			<td></td>
			<td>200</td>
			<td>64</td>
		</tr>
		<tr>
			<td></td>
			<td>50</td>
			<td>10</td>
		</tr>
		<tr>
			<td>BrocApt</td>
			<td>100</td>
			<td>20</td>
		</tr>
		<tr>
			<td></td>
			<td>150</td>
			<td>30</td>
		</tr>
		<tr>
			<td></td>
			<td>200</td>
			<td>40</td>
		</tr>
	</tbody>
</table>
Table 1: DNA copy number per capsule for each DNA design. The copy number was calculated according to Equation 1 and was correlated to the initial concentration of DNA plasmids, retention affinity of DNA sequences during the LbL deposition process, and the length of DNA plasmids.
<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Mw of FITC-Dextran (kDa)</td>
			<td>Hydrodynamic Radius (nm)</td>
		</tr>
		<tr>
			<td>4</td>
			<td>1.4</td>
		</tr>
		<tr>
			<td>20</td>
			<td>3.3</td>
		</tr>
		<tr>
			<td>40</td>
			<td>4.5</td>
		</tr>
		<tr>
			<td>70</td>
			<td>6</td>
		</tr>
		<tr>
			<td>150</td>
			<td>8.5</td>
		</tr>
		<tr>
			<td>250</td>
			<td>10</td>
		</tr>
		<tr>
			<td>500</td>
			<td>14</td>
		</tr>
		<tr>
			<td>2,000</td>
			<td>18</td>
		</tr>
	</tbody>
</table>
Table 2: Physical properties of FITC-dextrans. Hydrodynamic radius for each fluorophore was used to estimate the permeability of hollow silk fibroin microcapsules.

Watch this Scientific Journal Video about Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches at JoVE.com

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

The protocol describes the formation of robust and biocompatible DNA-laden microcapsules as multiplexed in vitro biosensors capable of tracking several ligands.

We introduce a protocol for the preparation of DNA-laden silk fibroin microcapsules via the Layer-by-Layer (LbL) assembly method on sacrificial spherical ...

preparation-multifunctional-silk-based-microcapsules-loaded-with-dna

Nanyang Technological University

Research

JoVE Journal

Biology

1.3K Views.  UES Inc.. The protocol describes the formation of robust and biocompatible DNA-laden microcapsules as multiplexed in vitro biosensors capable of tracking several ligands.

Video: Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Homemade Site Directed Mutagenesis of Whole Plasmids

Site directed mutagenesis of whole plasmids is a simple way to create slightly different variations of an original plasmid. With this method the cloned target gene can be altered by substitution, deletion or insertion of a few bases directly into a plasmid. It works by simply amplifying the whole plasmid, in a non PCR-based thermocycling reaction. During the reaction mutagenic primers, carrying the desired mutation, are integrated into the newly synthesized plasmid. In this video tutorial we demonstrate an easy and cost effective way to introduce base substitutions into a plasmid. The protocol works with standard reagents and is independent from commercial kits, which often are very expensive. Applying this protocol can reduce the total cost of a reaction to an eighth of what it costs using some of the commercial kits. In this video we also comment on critical steps during the process and give detailed instructions on how to design the mutagenic primers.

Site directed mutagenesis of whole plasmids is a simple way to create slightly different variations of an original plasmid. Here we demonstrate an easy and cost effective way to introduce base substitutions into a plasmid using standard reagents.

Site directed mutagenesis of whole plasmids is a simple way to create slightly different variations of an original plasmid. With this method the cloned ...

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

We describe a simple fluorescence microscopy-based real-time method for observing DNA replication at the single-molecule level. A circular, forked DNA template is attached to a functionalized glass coverslip and replicated extensively after introduction of replication proteins and nucleotides (Figure 1). The growing product double-strand DNA (dsDNA) is extended with laminar flow and visualized by using an intercalating dye. Measuring the position of the growing DNA end in real time allows precise determination of replication rate (Figure 2). Furthermore, the length of completed DNA products reports on the processivity of replication. This experiment can be performed very easily and rapidly and requires only a fluorescence microscope with a reasonably sensitive camera.


This protocol demonstrates a simple single-molecule fluorescence microscopy technique for visualizing DNA replication by individual replisomes in real time.

We describe a simple fluorescence microscopy-based real-time method for observing DNA replication at the single-molecule level. A circular, forked DNA ...

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Synthetic short nucleic acid polymers, oligonucleotides (oligos), are the most functional and widespread tools of molecular biology. Oligos can be produced to contain any desired DNA or RNA sequence and can be prepared to include a wide variety of base and sugar modifications. Moreover, oligos can be designed to mimic specific nucleic acid alterations and thus, can serve as important tools to investigate effects of DNA damage and mechanisms of repair. We found that Thermo Scientific Dharmacon RNA-containing oligos with a length between 50 and 80 nucleotides can be particularly suitable to study, in vivo, functions and consequences of chromosomal RNA/DNA hybrids and of ribonucleotides embedded into DNA. RNA/DNA hybrids can readily form during DNA replication, repair and transcription, however, very little is known about the stability of RNA/DNA hybrids in cells and to which extent these hybrids can affect the genetic integrity of cells. RNA-containing oligos, therefore, represent a perfect vector to introduce ribonucleotides into chromosomal DNA and generate RNA/DNA hybrids of chosen length and base composition. Here we present the protocol for the incorporation of ribonucleotides into the genome of the eukaryotic model system yeast /Saccharomyces cerevisiae/. Yet, our lab has utilized Thermo Scientific Dharmacon RNA-containing oligos to generate RNA/DNA hybrids at the chromosomal level in different cell systems, from bacteria to human cells.

This work shows how to form an RNA/DNA hybrid at the chromosomal level and reveal transfer of genetic information from RNA to genomic DNA in yeast cells.

Synthetic short nucleic acid polymers, oligonucleotides (oligos), are the most functional and widespread tools of molecular biology. Oligos can be ...

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

The unique composition and spatial arrangement of RNA-binding proteins (RBPs) on a transcript guide the diverse aspects of post-transcriptional regulation1. Therefore, an essential step towards understanding transcript regulation at the molecular level is to gain positional information on the binding sites of RBPs2.
Protein-RNA interactions can be studied using biochemical methods, but these approaches do not address RNA binding in its native cellular context. Initial attempts to study protein-RNA complexes in their cellular environment employed affinity purification or immunoprecipitation combined with differential display or microarray analysis (RIP-CHIP)3-5. These approaches were prone to identifying indirect or non-physiological interactions6. In order to increase the specificity and positional resolution, a strategy referred to as CLIP (UV cross-linking and immunoprecipitation) was introduced7,8. CLIP combines UV cross-linking of proteins and RNA molecules with rigorous purification schemes including denaturing polyacrylamide gel electrophoresis. In combination with high-throughput sequencing technologies, CLIP has proven as a powerful tool to study protein-RNA interactions on a genome-wide scale (referred to as HITS-CLIP or CLIP-seq)9,10. Recently, PAR-CLIP was introduced that uses photoreactive ribonucleoside analogs for cross-linking11,12.
Despite the high specificity of the obtained data, CLIP experiments often generate cDNA libraries of limited sequence complexity. This is partly due to the restricted amount of co-purified RNA and the two inefficient RNA ligation reactions required for library preparation. In addition, primer extension assays indicated that many cDNAs truncate prematurely at the crosslinked nucleotide13. Such truncated cDNAs are lost during the standard CLIP library preparation protocol. We recently developed iCLIP (individual-nucleotide resolution CLIP), which captures the truncated cDNAs by replacing one of the inefficient intermolecular RNA ligation steps with a more efficient intramolecular cDNA circularization (Figure 1)14. Importantly, sequencing the truncated cDNAs provides insights into the position of the cross-link site at nucleotide resolution. We successfully applied iCLIP to study hnRNP C particle organization on a genome-wide scale and assess its role in splicing regulation14.

The spatial arrangement of RNA-binding proteins on a transcript is a key determinant of post-transcriptional regulation. Therefore, we developed individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) that allows precise genome-wide mapping of the binding sites of an RNA-binding protein.

The unique composition and spatial arrangement of RNA-binding proteins (RBPs) on a transcript guide the diverse aspects of post-transcriptional ...

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

In vitro transcription is the synthesis of RNA transcripts by RNA polymerase from a linear DNA template containing the corresponding promoter sequence (T7, T3, SP6) and the gene to be transcribed (Figure 1A). A typical transcription reaction consists of the template DNA, RNA polymerase, ribonucleotide triphosphates, RNase inhibitor and buffer containing Mg2+ ions.
Large amounts of high quality RNA are often required for a variety of applications. Use of in vitro transcription has been reported for RNA structure and function studies such as splicing1, RNAi experiments in mammalian cells2, antisense RNA amplification by the "Eberwine method"3, microarray analysis4 and for RNA vaccine studies5. The technique can also be used for producing radiolabeled and dye labeled probes6. Warren, et al. recently reported reprogramming of human cells by transfection with in vitro transcribed capped RNA7. The T7 High Yield RNA Synthesis Kit from New England Biolabs has been designed to synthesize up to 180 &mu;g RNA per 20 &mu;l reaction. RNA of length up to 10kb has been successfully transcribed using this kit. Linearized plasmid DNA, PCR products and synthetic DNA oligonucleotides can be used as templates for transcription as long as they have the T7 promoter sequence upstream of the gene to be transcribed.
Addition of a 5' end cap structure to the RNA is an important process in eukaryotes. It is essential for RNA stability8, efficient translation9, nuclear transport10 and splicing11. The process involves addition of a 7-methylguanosine cap at the 5' triphosphate end of the RNA. RNA capping can be carried out post-transcriptionally using capping enzymes or co-transcriptionally using cap analogs. In the enzymatic method, the mRNA is capped using the Vaccinia virus capping enzyme12,13. The enzyme adds on a 7-methylguanosine cap at the 5' end of the RNA using GTP and S-adenosyl methionine as donors (cap 0 structure). Both methods yield functionally active capped RNA suitable for transfection or other applications14 such as generating viral genomic RNA for reverse-genetic systems15 and crystallographic studies of cap binding proteins such as eIF4E16.
In the method described below, the T7 High Yield RNA Synthesis Kit from NEB is used to synthesize capped and uncapped RNA transcripts of Gaussia luciferase (GLuc) and Cypridina luciferase (CLuc). A portion of the uncapped GLuc RNA is capped using the Vaccinia Capping System (NEB). A linearized plasmid containing the GLuc or CLuc gene and T7 promoter is used as the template DNA. The transcribed RNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity. Capped CLuc RNA is used as the internal control to normalize GLuc expression.

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.

In vitro transcription is the synthesis of RNA transcripts by RNA polymerase from a linear DNA template containing the corresponding promoter sequence ...

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Mechanobiology is an emerging scientific area that addresses the critical role of physical cues in directing cell morphology and function. For example, the effect of tissue elasticity on cell function is a major area of mechanobiology research because tissue stiffness modulates with disease, development, and injury. Static tissue-mimicking materials, or materials that cannot alter stiffness once cells are plated, are predominately used to investigate the effects of tissue stiffness on cell functions. While information gathered from static studies is valuable, these studies are not indicative of the dynamic nature of the cellular microenvironment in vivo. To better address the effects of dynamic stiffness on cell function, we developed a DNA-crosslinked polyacrylamide hydrogel system (DNA gels). Unlike other dynamic substrates, DNA gels have the ability to decrease or increase in stiffness after fabrication without stimuli. DNA gels consist of DNA crosslinks that are polymerized into a polyacrylamide backbone. Adding and removing crosslinks via delivery of single-stranded DNA allows temporal, spatial, and reversible control of gel elasticity. We have shown in previous reports that dynamic modulation of DNA gel elasticity influences fibroblast and neuron behavior. In this report and video, we provide a schematic that describes the DNA gel crosslinking mechanisms and step-by-step instructions on the preparation DNA gels.

Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.

Mechanobiology is an emerging scientific area that addresses the critical role of physical cues in directing cell morphology and function. For example, ...

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

The growing realization that both the temporal and spatial regulation of gene expression can have important consequences on cell function has led to the development of diverse techniques to visualize individual RNA transcripts in single living cells. One promising technique that has recently been described utilizes an oligonucleotide-based optical probe, ratiometric bimolecular beacon (RBMB), to detect RNA transcripts that were engineered to contain at least four tandem repeats of the RBMB target sequence in the 3&#8217;-untranslated region. RBMBs are specifically designed to emit a bright fluorescent signal upon hybridization to complementary RNA, but otherwise remain quenched. The use of a synthetic probe in this approach allows photostable, red-shifted, and highly emissive organic dyes to be used for imaging. Binding of multiple RBMBs to the engineered RNA transcripts results in discrete fluorescence spots when viewed under a wide-field fluorescent microscope. Consequently, the movement of individual RNA transcripts can be readily visualized in real-time by taking a time series of fluorescent images. Here we describe the preparation and purification of RBMBs, delivery into cells by microporation and live-cell imaging of single RNA transcripts.

Ratiometric bimolecular beacons (RBMBs) can be used to image single engineered RNA transcripts in living cells. Here, we describe the preparation and purification of RBMBs, delivery of RBMBs into cells by microporation and fluorescent imaging of single RNA transcripts in real-time.

The growing realization that both the temporal and spatial regulation of gene expression can have important consequences on cell function has led to the ...

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

Until recently, astronaut blood samples were collected in-flight, transported to earth on the Space Shuttle, and analyzed in terrestrial laboratories. If humans are to travel beyond low Earth orbit, a transition towards space-ready, point-of-care (POC) testing is required. Such testing needs to be comprehensive, easy to perform in a reduced-gravity environment, and unaffected by the stresses of launch and spaceflight. Countless POC devices have been developed to mimic laboratory scale counterparts, but most have narrow applications and few have demonstrable use in an in-flight, reduced-gravity environment. In fact, demonstrations of biomedical diagnostics in reduced gravity are limited altogether, making component choice and certain logistical challenges difficult to approach when seeking to test new technology. To help fill the void, we are presenting a modular method for the construction and operation of a prototype blood diagnostic device and its associated parabolic flight test rig that meet the standards for flight-testing onboard a parabolic flight, reduced-gravity aircraft. The method first focuses on rig assembly for in-flight, reduced-gravity testing of a flow cytometer and a companion microfluidic mixing chip. Components are adaptable to other designs and some custom components, such as a microvolume sample loader and the micromixer may be of particular interest. The method then shifts focus to flight preparation, by offering guidelines and suggestions to prepare for a successful flight test with regard to user training, development of a standard operating procedure (SOP), and other issues. Finally, in-flight experimental procedures specific to our demonstrations are described.

Spaceflight blood diagnostics need innovation. Few demonstrations have been published illustrating in-flight, reduced-gravity health diagnostic technology. Here we present a method for construction and operation of a parabolic flight test rig for a prototype point-of-care flow-cytometry design, with components and preparation strategies adaptable to other setups.

Until recently, astronaut blood samples were collected in-flight, transported to earth on the Space Shuttle, and analyzed in terrestrial laboratories. If ...

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Retroviruses exhibit signature integration preferences on both the local and global scales. Here, we present a detailed protocol for (1) generation of diverse libraries of retroviral integration sites using ligation-mediated PCR (LM-PCR) amplification and next-generation sequencing (NGS), (2) mapping the genomic location of each virus-host junction using BEDTools, and (3) analyzing the data for statistical relevance. Genomic DNA extracted from infected cells is fragmented by digestion with restriction enzymes or by sonication. After suitable DNA end-repair, double-stranded linkers are ligated onto the DNA ends, and semi-nested PCR is conducted using primers complementary to both the long terminal repeat (LTR) end of the virus and the ligated linker DNA. The PCR primers carry sequences required for DNA clustering during NGS, negating the requirement for separate adapter ligation. Quality control (QC) is conducted to assess DNA fragment size distribution and adapter DNA incorporation prior to NGS. Sequence output files are filtered for LTR-containing reads, and the sequences defining the LTR and the linker are cropped away. Trimmed host cell sequences are mapped to a reference genome using BLAT and are filtered for minimally 97% identity to a unique point in the reference genome. Unique integration sites are scrutinized for adjacent nucleotide (nt) sequence and distribution relative to various genomic features. Using this protocol, integration site libraries of high complexity can be constructed from genomic DNA in three days. The entire protocol that encompasses exogenous viral infection of susceptible tissue culture cells to integration site analysis can therefore be conducted in approximately one to two weeks. Recent applications of this technology pertain to longitudinal analysis of integration sites from HIV-infected patients.

We describe a protocol for amplifying retroviral integration sites from the genomic DNA of infected cells, sequencing the amplified virus-host junctions, and then mapping these sequences to a reference genome. We also describe techniques to quantify the distribution of integration sites relative to various genomic annotations using BEDTools.

Retroviruses exhibit signature integration preferences on both the local and global scales. Here, we present a detailed protocol for (1) generation of ...

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Large DNA molecules tethered on the functionalized glass surface have been utilized in polymer physics and biochemistry particularly for investigating interactions between DNA and its binding proteins. Here, we report a method that uses fluorescent microscopy for visualizing large DNA molecules tethered on the surface. First, glass coverslips are biotinylated and passivated by coating with biotinylated polyethylene glycol, which specifically binds biotinylated DNA via avidin protein linkers and significantly reduces undesirable binding from non-specific interactions of proteins or DNA molecules on the surface. Second, the DNA molecules are biotinylated by two different methods depending on their terminals. The blunt ended DNA is tagged with biotinylated dUTP at its 3' hydroxyl terminus, by terminal transferase, while the sticky ended DNA is hybridized with biotinylated complimentary oligonucleotides by DNA ligase. Finally, a microfluidic shear flow makes single DNA molecules stretch to their full contour lengths after being stained with fluorescent protein-DNA binding peptide (FP-DBP).

We present an approach for visualizing fluorescent protein DNA binding peptide (FP-DBP)-stained large DNA molecules tethered on the polyethylene glycol (PEG) and avidin-coated glass surface and stretched with microfluidic shear flows.

Large DNA molecules tethered on the functionalized glass surface have been utilized in polymer physics and biochemistry particularly for investigating ...