Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

0:56

Tissue Sample Collection and Fixation

2:04

Paraffin Infiltration and Embedding

2:59

Preparation of Slides for Staining and Host Features

4:31

Bacterial Staining with FISH

6:41

Results: Analysis of the Localization of Muribaculum intestinale Relative to Mucus in a Gnotobiotic Mouse Model and Some Common Imaging Problems

8:33

Conclusion