JoVE Journal
Immunology and Infection
This content is Open Access.
Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging
7.9K Views
•
09:31 min
•
July 9th, 2021
DOI :
July 9th, 2021
•Chapters in this video
0:04
Introduction
0:56
Tissue Sample Collection and Fixation
2:04
Paraffin Infiltration and Embedding
2:59
Preparation of Slides for Staining and Host Features
4:31
Bacterial Staining with FISH
6:41
Results: Analysis of the Localization of Muribaculum intestinale Relative to Mucus in a Gnotobiotic Mouse Model and Some Common Imaging Problems
8:33
Conclusion
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ISSN 2689-3649
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