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Nanyang Technological University

Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

1.8K Views

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07:17 min

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April 26th, 2021

DOI :

10.3791/62352-v

April 26th, 2021

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Janet Y. Sheung¹, Megan Otsuka², Gabriella Seifert³, Athena Lin⁴, Wallace F. Marshall⁴

¹W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, ²W. M. Keck Science Department, Pitzer College, ³W. M. Keck Science Department, Scripps College, ⁴Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco

Summary

We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.

Explore More Videos

Stentor Coeruleus

Motility Patterns

Unicellular Regeneration

Oral Apparatus Regeneration

Cell Tracking Assay

Imaging Chamber Construction

Silicone Spacer Sheets

Time Lapse Video

Microscope Calibration

Data Organization

Sample Preparation

Regeneration Timeline

Functional Regeneration

Chapters in this video

0:05

Introduction

1:03

Sample Preparation

2:15

Visible Light Microscopy Time-Lapse

2:56

Cell Tracking

3:52

Trace Verification

4:26

Data Visualization

5:14

Results: Motility Patterns of Stentor During and After Oral Apparatus

6:34

Conclusion

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