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Nanyang Technological University

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Mechanical forces are important for controlling cell migration. This protocol demonstrates the use of elastic hydrogels that can be deformed using a glass micropipette and a micromanipulator to stimulate cells with a local stiffness gradient to elicit changes in cell structure and migration.

Chapters in this video

0:04

Title

0:41

Glass Coverslip Activation

1:15

Fluorescent Microbead Deposition and Hydrogel Casting

2:38

Hydrogel Activation and Extracellular Matrix Coating

3:38

Cell Plating and Micropipette Preparation

5:51

Micromanipulator Calibration and Durotaxis Assay

7:01

Results: Representative Durotactic Stimulation Responses and Protein Expression Analyses

7:55

Conclusion

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